PMID: 9190204Feb 1, 1997Paper

Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association

Molecular Biology of the Cell
R F ZirwesM S Schmidt-Zachmann

Abstract

To identify the element(s) in nucleolar proteins which determine nucleolus-specific topogenesis, we have used different kinds of cDNA constructs encoding various chimeric combinations of mutants of the constitutive nucleolar protein NO38 (B23): 1) with an amino terminally placed short "myc tag"; 2) with two different carboxyl terminally attached large alpha-helical coiled coil structures, the lamin A rod domain or the rod domain of vimentin; 3) with the sequence-related nucleoplasmic histone-binding protein nucleo-plasmin; and 4) with the soluble cytoplasmic protein pyruvate kinase. To avoid the problem of formation of complexes with endogenous wild-type (wt) molecules and "piggyback" localization, special care was taken to secure that the mutants and chimeras used did not oligomerize as is typical of protein NO38 (B23). Using microinjection and transfection of cultured cells, we found that the segment comprising the amino-terminal 123 amino acids (aa) alone was sufficient to effect nucleolar accumulation of the construct molecules, including the chimeras with the entire rod domains of lamin A and vimentin. However, when the amino-terminal 109 aa were deleted, the molecules still associated with the nucleolus. The results of fu...Continue Reading

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Citations

Jun 11, 2011·International Journal of Hematology·Masafumi YamaguchiNaoki Okamura
Mar 14, 1998·Molecular Biology of the Cell·M S Schmidt-ZachmannA Krämer
Jan 27, 2004·Molecular Biology of the Cell·Jens EilbrachtMarion S Schmidt-Zachmann
Jan 24, 1998·Nucleic Acids Research·D UrsicM R Culbertson
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·J Eilbracht, M S Schmidt-Zachmann
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·R F ZirwesW W Franke
Nov 23, 2006·Cancer Detection and Prevention·Mi Jung Lim, Xin Wei Wang
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Jun 28, 2005·Biochemical and Biophysical Research Communications·Edgar LipsiusJoachim Kruppa
Aug 1, 1997·Differentiation; Research in Biological Diversity·R Paffenholz, W W Franke
Dec 15, 2010·Biochemistry Research International·Mikael S Lindström
Aug 6, 2011·Nucleus·Danièle Hernandez-Verdun
Dec 4, 2004·Structure·V M Haridasan NamboodiriChristopher W Akey
Jan 24, 2002·Molecular Cell·Miroslav Dundr, Tom Misteli
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Jun 6, 2003·The Journal of Biological Chemistry·Bryan C JensenMarilyn Parsons
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