Topographic differences in cell populations and insulin secretion in the endocrine pancreas of the toad Bufo arenarum

General and Comparative Endocrinology
F FranciniJ J Gagliardino

Abstract

We analyzed the endocrine cell topography within the amphibian pancreas and the relationship of this distribution to lobular variation in insulin content and secretion. Pancreases from adult male toad Bufo arenarum were separated into their five lobes: free, gastric, hepatic, duodenal, and jejunal. Pieces of each lobe were incubated with glucose, arginine, and K+ and the insulin concentration in the medium was measured by radioimmunoassay. In the presence of 2 or 8 mM glucose, 10 mM arginine, and 10 mM K+ the free lobe released a significantly greater amount of insulin than the other lobes, while the output of the gastric lobe was greater than that of the duodenal, hepatic, and jejunal. At 8 mM glucose, every pancreatic lobe released a significantly higher amount of insulin than at 2 mM. The insulin content of the free lobe was significantly higher than that of the others, whereas this parameter was comparable among the latter. These pancreases contained islets of variable size and irregular shape. B and non-B cells, detected by immunoperoxidase staining, were located at the central and peripheral zones of the islets, respectively. A large number of non-B cells were also scattered over the exocrine component. Morphometrical ana...Continue Reading

Citations

Mar 9, 2000·Diabetes/metabolism Research and Reviews·J J Gagliardino
Apr 15, 2000·General and Comparative Endocrinology·J C EtayoA C Villaro
Apr 20, 1999·General and Comparative Endocrinology·M T LozanoB Agulleiro

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