Topographic refinement of the regenerating retinotectal projection of the goldfish in standard laboratory conditions: a quantitative WGA-HRP study

Experimental Brain Research
E C Rankin, J E Cook

Abstract

The topographic precision of the regenerating retinotectal projection of the goldfish was studied between 18 and 524 days (at 20 degrees C) after optic nerve cut, using retrograde transport of wheatgerm agglutinin conjugated to horseradish peroxidase (WGA-HRP) from one of two standardized tectal injection sites. All labelled ganglion cells in each flat-mounted retina were plotted individually, and their degree of dispersion was assessed by a statistical method based on distance to nearest neighbour. Labelled cells in normal fish were clustered tightly, covering on average only 1.3% of the retina. Early in regeneration (18-28 days) they were widely dispersed, covering up to 75.2%, and they did not begin to form recognizable clusters at appropriate sites until about 35 days after nerve cut. Between 18 and 70 days, the proportion of retina covered by labelled cells fell dramatically, halving about every 14 days. Between 70 and 524 days, no further reduction could be demonstrated: overall, clusters remained significantly larger than normal, though a few individual retinae were virtually normal. Several others, labelled from similar single injections between 56 and 524 days after nerve cut, showed pairs of cell clusters; a sign that...Continue Reading

Citations

Apr 1, 1992·Neurochemistry International·H RahmannS Esders
Feb 1, 1990·The European Journal of Neuroscience·Jeremy E. Cook, David L. Becker
Oct 1, 1989·Neuron·H T Cline, M Constantine-Paton
Aug 22, 1990·Proceedings. Biological Sciences·D L Becker, J E Cook
May 1, 2001·The Journal of Comparative Neurology·A J Dawson, R L Meyer
Oct 22, 1989·The Journal of Comparative Neurology·U Busse, C A Stuermer
Jun 1, 1989·The Journal of Comparative Neurology·R Bernhardt
Jun 1, 1989·The Journal of Comparative Neurology·E Hartlieb, C A Stuermer
Oct 22, 1991·The Journal of Comparative Neurology·R L Levine

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