Topographical alterations in proteins I of Neisseria gonorrhoeae correlated with lipooligosaccharide variation

Molecular Microbiology
R C Judd, W M Shafer

Abstract

Four transformant strains of Neisseria gonorrhoeae were generated, two of which (WS3 and WS5) had protein I subclass A (P.IA) and two which (WS2 and WS4) had protein I subclass B (P.IB). Analysis of the strains demonstrated that the two P.IA-bearing strains differed in lipooligosaccharide (LOS) and H.8 antigen, as assessed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The WS5 strain had slow-migrating LOS and H.8 antigen, and the WS3 strain had fast-migrating LOS and H.8 antigen. The P.IB-bearing strains also had either slow-migrating LOS and H.8 antigen (WS4) or fast-migrating LOS and H.8 antigen (WS2). Structural and exposure analysis revealed that although the P.IAs were identical in the WS3 and WS5 strains, there was a slight alteration of the exposure of the proteins which correlated with altered LOS and/or H.8 antigen. The P.IBs were also shown to be structurally identical, but the LOS and/or H.8 antigen variation in these strains correlated with a more pronounced alteration in the exposure of the P.IB molecules. The differences in protein I (P.I) exposure were generally found in highly negatively charged regions of the molecule, suggesting that the immunogenicity and/or anti...Continue Reading

References

Feb 1, 1986·The Journal of Infectious Diseases·P F SparlingM So
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S BergströmJ Swanson
May 1, 1986·The Journal of Infectious Diseases·W M ShaferP J Hitchcock
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Dec 1, 1983·The Journal of Experimental Medicine·A G BarbourR C Judd
May 1, 1981·Infection and Immunity·J G CannonP F Sparling
Jul 1, 1982·Infection and Immunity·J Swanson
Dec 1, 1982·Infection and Immunity·H D Caldwell, R C Judd

❮ Previous
Next ❯

Citations

May 8, 1990·Journal of Immunological Methods·R K PettitR C Judd
Nov 13, 2003·BMC Microbiology·Philip JordanNigel J Saunders
Aug 1, 1990·Molecular Microbiology·W M ShaferL W Huthwaite

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.