Topography and refractometry of sperm cells using spatial light interference microscopy

Journal of Biomedical Optics
Lina LiuGabriel Popescu

Abstract

Characterization of spermatozoon viability is a common test in treating infertility. Recently, it has been shown that label-free, phase-sensitive imaging can provide a valuable alternative for this type of assay. We employ spatial light interference microscopy (SLIM) to perform high-accuracy single-cell phase imaging and decouple the average thickness and refractive index information for the population. This procedure was enabled by quantitative-phase imaging cells on media of two different refractive indices and using a numerical tool to remove the curvature from the cell tails. This way, we achieved ensemble averaging of topography and refractometry of 100 cells in each of the two groups. The results show that the thickness profile of the cell tail goes down to 150 nm and the refractive index can reach values of 1.6 close to the head.

References

Jul 1, 1964·The American Journal of Anatomy·R G SAACKE, J O ALMQUIST
Aug 19, 2007·Nature Methods·Wonshik ChoiMichael S Feld
May 5, 2009·Optics Letters·Huafeng DingGabriel Popescu
Jan 26, 2011·Optics Express·Zhuo WangGabriel Popescu
Apr 12, 2011·Optics Letters·Zhuo WangGabriel Popescu
Oct 15, 2011·Optics Express·Zhuo WangGabriel Popescu
Nov 25, 2011·Journal of Biomedical Optics·Zhuo WangGabriel Popescu
Apr 4, 2015·Biosensors·Maria Antonietta FerraraAnna Chiara De Luca
May 1, 2015·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalMichelle Renee Chyatte
Jan 12, 2016·Journal of Human Reproductive Sciences·Naina Kumar, Amit Kant Singh
Mar 25, 2016·Journal of Biophotonics·Mirjam SchürmannChii J Chan
May 27, 2016·Journal of Biomedical Optics·Raksha RaghunathanKirill V Larin
Jun 28, 2017·Journal of Biomedical Optics·Mikhail E KandelGabriel Popescu
Aug 9, 2017·Nature Communications·Tan H NguyenGabriel Popescu
Mar 20, 2018·Biomedical Optics Express·Mikhail E KandelGabriel Popescu

❮ Previous
Next ❯

Citations

Feb 19, 2020·Systems Biology in Reproductive Medicine·Marcello RubessaMatthew B Wheeler
Apr 18, 2020·Science Advances·Gili Dardikman-YoffeNatan T Shaked
May 18, 2021·Biomedical Optics Express·Rongli GuoNatan T Shaked

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.