Topography induces differential sensitivity on cancer cell proliferation via Rho-ROCK-Myosin contractility

Scientific Reports
Parthiv Kant ChaudhuriChwee Teck Lim

Abstract

Although the role of stiffness on proliferative response of cancer cells has been well studied, little is known about the effect of topographic cues in guiding cancer cell proliferation. Here, we examined the effect of topographic cues on cancer cell proliferation using micron scale topographic features and observed that anisotropic features like microgratings at specific dimension could reduce proliferation of non-cancer breast epithelial cells (MCF-10A) but not that for malignant breast cancer cells (MDA-MB-231 and MCF-7). However, isotropic features such as micropillars did not affect proliferation of MCF-10A, indicating that the anisotropic environmental cues are essential for this process. Interestingly, acto-myosin contraction inhibitory drugs, Y-27632 and blebbistatin prevented micrograting-mediated inhibition on proliferation. Here, we propose the concept of Mechanically-Induced Dormancy (MID) where topographic cues could activate Rho-ROCK-Myosin signaling to suppress non-cancerous cells proliferation whereas malignant cells are resistant to this inhibitory barrier and therefore continue uncontrolled proliferation.

References

Apr 23, 1999·Biomaterials·R G FlemmingP F Nealey
Jun 14, 2003·Toxicological Sciences : an Official Journal of the Society of Toxicology·Susan L StarcevicRaymond F Novak
Apr 9, 2005·Biomaterials·Evelyn K F YimKam W Leong
Apr 22, 2006·Cellular Signalling·Wolfgang F VogelCaroline E Ford
Oct 10, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Wendy V IngmanJeffrey W Pollard
Nov 18, 2006·Cell·Gaorav P Gupta, Joan Massagué
Dec 28, 2006·BMC Medicine·Paolo P ProvenzanoPatricia J Keely
Jan 19, 2007·The New England Journal of Medicine·Norman F BoydMartin J Yaffe
Feb 2, 2008·Biomaterials·Esther RebollarJohannes Heitz
Sep 17, 2008·Cancer Research·Peter Friedl, Katarina Wolf
Mar 25, 2009·Nature Reviews. Cancer·Don X NguyenJoan Massagué
Nov 26, 2009·Cell·Kandice R LeventalValerie M Weaver
Mar 2, 2011·The American Journal of Pathology·Matthew W ConklinPatricia J Keely
Oct 12, 2011·Journal of Biomedical Materials Research. Part a·Lijuan Zhang, Thomas J Webster
Oct 26, 2012·Journal of Cell Science·Justin D MihDaniel J Tschumperlin
Mar 4, 2014·Materials Science & Engineering. C, Materials for Biological Applications·R OrtizJ L Toca-Herrera
Dec 4, 2014·Biophysical Journal·Kristin M RichingPatricia J Keely

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Citations

Apr 14, 2017·Scientific Reports·Hiroaki HirataMasahiro Sokabe
Jun 8, 2017·Journal of Functional Biomaterials·Ryota DomuraMasami Okamoto
Aug 10, 2017·Journal of Functional Biomaterials·Chunga KwonHojeong Jeon
Nov 17, 2017·Nature Communications·Wang XiChwee Teck Lim
Jun 8, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bing ChenKaizhi Lu
Jan 17, 2021·Scientific Reports·Aysegul Dede ErenJasper Foolen
Jan 2, 2021·Journal of Biomechanics·Victoria L ThaiJ Kent Leach
Sep 14, 2020·Experimental Eye Research·W Matthew PetrollDavid W Schmidtke
Oct 31, 2020·Science·Hadi T NiaRakesh K Jain
Jan 7, 2021·Annals of Biomedical Engineering·Farbod AmirghasemiMehdi Nikkhah
Jun 12, 2020·Acta Biomaterialia·Fatemeh ShahriyariAli Khademhosseini
Jun 22, 2018·Chemical Reviews·Parthiv Kant ChaudhuriChwee Teck Lim
Aug 12, 2019·ACS Biomaterials Science & Engineering·GeonHui LeeDong-Hwee Kim
Sep 21, 2021·Physiological Reviews·Eva Crosas-MolistVictoria Sanz-Moreno

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

ImageJ

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