Topography of amphiregulin expression in cultured human keratinocytes: colocalization with the epidermal growth factor receptor and CD44

In Vitro Cellular & Developmental Biology. Animal
N NylanderM Piepkorn

Abstract

Much of the autonomous growth of cultured keratinocytes is attributable to the signaling of amphiregulin, a heparin-binding autocrine growth factor, through the epidermal growth factor receptor. Emerging evidence suggests, moreover, that the membrane proteoglycan, CD44, is a cofactor for the interaction of heparin-binding ligands with their receptors. This model was evaluated by characterizing the patterns of the immunolabeled molecules in cultured human neonatal keratinocytes, to test the hypothesis that involvement in a common function results in coordinate segregation within or on the cell. The molecules were localized by double immunofluorescence labeling to detect amphiregulin and either the epidermal growth factor receptor or CD44, and the immunostained products were imaged by scanning laser confocal microscopy. Both amphiregulin and the epidermal growth factor receptor segregated to a perinuclear distribution and to intercellular contacts. In addition, amphiregulin localized to the outer leading edge of colonies and focally to intranuclear sites. Metabolic blockade of proteoglycan sulfation with sodium chlorate inhibited growth of the cells and concurrently enhanced the nuclear, but decreased the outer leading edge, labe...Continue Reading

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Citations

Apr 26, 2008·Journal of Mammary Gland Biology and Neoplasia·Nicole E Willmarth, Stephen P Ethier
Mar 22, 2003·Experimental Cell Research·Raymond C HarrisRobert J Coffey
Feb 11, 2012·Cancer Science·Michihiro YoshidaTakashi Joh
Feb 14, 2014·PloS One·Seungwon Yang, Hyun-Man Kim
Nov 17, 2020·Frontiers in Medicine·Mahmood Yaseen HachimRifat Hamoudi
Apr 17, 2012·Journal of Cell Science·E Douglas RobertsonAndrey A Panteleyev

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