PMID: 7518858Aug 1, 1994Paper

Topography of mammalian connexins in human skin

The Journal of Investigative Dermatology
D SalomonP Meda

Abstract

We have explored the expression of gap junction proteins in normal human skin by immunostaining cryostat sections (indirect immunofluorescence) or lyophilized epidermis (Western blotting) with antibodies against four mammalian connexins Cx26, Cx32, Cx40, Cx43; and by hybridizing total epidermal RNA (Northern blotting) with cRNA probes for Cx26, Cx32, and Cx43. We found that epidermal keratinocytes express Cx43 but not Cx26, Cx32, or Cx40. This expression was minimal in the basal layer, much higher in the spinous layer, reduced in the granular layer, and absent in the stratum corneum. Immunostaining for Cx43 was also observed in sebaceous glands, hairs, and eccrine sweat ducts. The two latter epidermal adnexae were also markedly labeled by antibodies against Cx26, a gap junction protein that was undetectable by immunofluorescence in interfollicular keratinocytes. Immunoblots of polyacrylamide gel electrophoresis-separated epidermal proteins and hybridization of epidermal RNA confirmed the presence of Cx43 in epidermis. These observations indicate that 1) Cx43 and Cx26 are components of human keratinocyte gap junctions; 2) these two proteins are differentially expressed in the interfollicular epidermis and the skin adnexae; 3) in...Continue Reading

References

Oct 1, 1977·Journal of Ultrastructure Research·R Caputo, D Peluchetti
Jun 19, 1992·International Journal of Cancer. Journal International Du Cancer·K K WilgenbusO Traub
Feb 1, 1992·Seminars in Cell Biology·N M Kumar, N B Gilula
Feb 1, 1992·Seminars in Cell Biology·A Warner
Feb 1, 1992·Seminars in Cell Biology·K A Stauffer, N Unwin
Feb 1, 1992·Seminars in Cell Biology·W R Loewenstein, B Rose
Mar 1, 1991·Neuron·M V BennettJ C Sáez
Jul 1, 1990·The Journal of Membrane Biology·E C BeyerD A Goodenough
Dec 1, 1990·The Journal of Cell Biology·E Fuchs
Dec 1, 1987·Experimental Cell Research·E KamJ D Pitts
Jul 1, 1988·The Journal of Clinical Investigation·D SalomonP Meda
Dec 1, 1989·Current Opinion in Cell Biology·F M Watt
Dec 1, 1987·The Journal of Cell Biology·E C BeyerD A Goodenough
Jul 1, 1986·The Journal of Cell Biology·D L Paul
Jul 1, 1971·The Journal of Investigative Dermatology·K Hashimoto
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Oct 1, 1972·Biophysical Journal·J S van HeukelomJ C van der Leun
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas

❮ Previous
Next ❯

Citations

Sep 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·E Masgrau-PeyaP Meda
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Nicolas ProvostCarine Nizard
Feb 24, 2004·The Journal of Biological Chemistry·Tamsin ThomasDale W Laird
Aug 7, 2009·Cell and Tissue Research·Moe TsutsumiMitsuhiro Denda
Nov 3, 2010·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Yun-Hoon ChoungSeung Joo Lee
Jul 2, 2010·Dermatology Research and Practice·Sirkku PeltonenJuha Peltonen
Oct 21, 2011·Physiological Reviews·Domenico BoscoPaolo Meda
Aug 15, 2012·Archives of Dermatological Research·Que-Ping LiuXiang Chen
Mar 27, 2018·American Journal of Medical Genetics. Part a·Desiree DeMilleJohn C Carey
May 15, 1996·European Journal of Biochemistry·R BruzzoneD L Paul
Dec 1, 1996·Journal of Cutaneous Pathology·J Tada, K Hashimoto
Jun 18, 2002·Cell Communication & Adhesion·L WiszniewskiP Meda
Nov 2, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Daniel GonzálezLuis C Barrio
Aug 22, 2006·Pediatric Dermatology·Stephen YooRonald Cotliar
Mar 20, 2004·The British Journal of Dermatology·K AritaH Shimizu
Mar 25, 2009·The Biochemical Journal·Joell L Solan, Paul D Lampe
May 3, 2013·Physiology·Jared M Churko, Dale W Laird
Nov 25, 2003·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Kay M AbdullahAnna T Grazul-Bilska
Nov 6, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ken AritaHiroshi Shimizu
Jan 24, 2006·Acta Oto-laryngologica·Yun-Hoon ChoungPill-Hoon Choung
Dec 23, 2003·The European Journal of Neuroscience·Mikhail A FilippovHannah Monyer
Apr 27, 2012·Cardiology Research and Practice·Josephine A WrightDavid L Becker
Feb 9, 2010·Pflügers Archiv : European journal of physiology·Georg Zoidl, Rolf Dermietzel
Jun 16, 2015·BioMed Research International·Ana Paula GrilloCamila Andréa de Oliveira
Sep 6, 2018·Cold Spring Harbor Perspectives in Medicine·Fabio Mammano
Jan 10, 2019·Frontiers in Oncology·Maksim SinyukJustin Lathia
Apr 27, 2004·American Journal of Medical Genetics. Part a·Klaus W KjaerNiels Tommerup
Feb 24, 2006·The Biochemical Journal·Dale W Laird
Jun 18, 2002·Cell Communication & Adhesion·G Richard
Mar 14, 2009·American Journal of Medical Genetics. Part a·Fabiana AlexandrinoEdi Lúcia Sartorato

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.