PMID: 6159920Nov 18, 1980Paper

Topography of phosphatidylcholine, phosphatidylethanolamine and triacylgycerol biosynthetic enzymes in rat liver microsomes

Biochimica Et Biophysica Acta
L M Ballas, R M Bell

Abstract

The topography of phosphatidylcholine, phosphatidylethanolamine and triacylglycerol biosynthetic enzymes within the transverse plane of rat liver microsomes was investigated using two impermeant inhibitors, mercury-dextran and dextran-maleimide. Between 70 and 98% of the activities of fatty acid : CoA ligase (EC 6.2.1.3), sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.15), phosphatidic acid phosphatase (EC 3.1.3.4), diacylglycerol acyltransferase (EC 2.3.1.20), diacylglycerol cholinephosphotransferase (EC 2.7.8.2) and diacylglycerol ethanolaminephosphotransferase (EC 2.7.8.1) were inactivated by mercury-dextran. Dextran-maleimide caused 52% inactivation of the sn-glycerol-3-phosphate acyltransferase. Inactivation of each of these activities except fatty acid : CoA ligase occurred in microsomal vesicles which remained intact as evidenced by the maintenance of highly latent mannose-6-phosphatase activity (EC 3.1.3.9). These glycerolipid biosynthetic activities were not latent, indicating that substrates have free access to the active sites. Moreover, ATP, CDP-choline and CMP appeared unable to penetrate the microsome membrane. These data indicate that the active sites of thease enzymes are located on the external surface of mi...Continue Reading

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Citations

Jan 1, 1986·The International Journal of Biochemistry·S PikułaM G Sarzała
Mar 13, 2009·Journal of Cellular and Molecular Medicine·Katherine A PriceCyril C Curtain
Feb 19, 1991·Biochemistry·R H Hjelmstad, R M Bell
May 4, 2000·Progress in Lipid Research·L Dircks, H S Sul
Jan 1, 1996·Progress in Lipid Research·R Lehner, A Kuksis
Nov 1, 1989·Chemistry and Physics of Lipids·W L Adair, N Cafmeyer
Sep 12, 1984·Biochimica Et Biophysica Acta·L M BallasR M Bell
Dec 6, 1984·Biochimica Et Biophysica Acta·P A Walton, F Possmayer
Nov 12, 1982·Biochimica Et Biophysica Acta·J M KesselsH van den Bosch
Apr 25, 1985·Biochimica Et Biophysica Acta·R A Coleman, E B Haynes

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