PMID: 7015289Feb 25, 1981Paper

Topography of the C. coli 5S RNA-protein complex as determined by crosslinking with dimethyl suberimidate and dimethyl-3,3'-dithiobispropionimidate

Nucleic Acids Research
T G Fanning, R R Traut

Abstract

5S RNA-protein complexes were prepared in vitro using partially purified E. coli 5S RNA and total E. coli 70S ribosomal proteins. The complexes were isolated from sucrose gradients and shown to contain proteins L5, L18, L25 and a fourth protein not heretofore characterized and designed L31. The complexes were treated with the crosslinking reagents dimethyl suberimidate and dimethyl-3,3'-dithiobispropionimidate. Both reagents gave identical patterns of crosslinked proteins when analyzed by one-dimensional polyacrylamide/dodecylsulfate gel electrophoresis. Dimers of L5-L31', L5-L18 and L18-L18 and a trimer containing L5, L18 and L31' were identified by diagonal polyacrylamide/dodecylsulfate gel electrophoresis of the proteins crosslinked with dimethyl-3,3'-dithiobispropionimidate. No crosslinking was detected between L25 and the other three proteins.

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Citations

Jul 30, 2009·Journal of Molecular Evolution·Feng-Jie Sun, Gustavo Caetano-Anollés
Nov 11, 1999·FEMS Microbiology Letters·A J EistetterT G Fanning
Nov 25, 1981·Nucleic Acids Research·H F NollerC R Waese
Apr 22, 2009·Research in Microbiology·Aviram Rasouly, Eliora Z Ron
Jan 1, 1982·Progress in Biophysics and Molecular Biology·A Liljas

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