Topography of the surface of the signal-transducing protein EIIA(Glc) that interacts with the MalK subunits of the maltose ATP-binding cassette transporter (MalFGK2) of Salmonella typhimurium

The Journal of Biological Chemistry
Bettina BlüschkeErwin Schneider

Abstract

The signal-transducing protein EIIA(Glc), a component of the phosphoenolpyruvate-glucose phosphotransferase system, plays a key role in carbon regulation in enteric bacteria, such as Escherichia coli and Salmonella typhimurium. The phosphorylation state of EIIA(Glc) governs transport and metabolism of a number of carbohydrates. When glucose as preferred carbon source is transported, EIIA(Glc) becomes predominantly unphosphorylated and allosterically inhibits several permeases, including the maltose ATP-binding cassette transport system (MalFGK2) in a process termed "inducer exclusion." We have mapped the binding surface of EIIA(Glc) that interacts with the MalK subunits by using synthetic cellulose-bound peptide arrays like pep scan- and substitutional analyses. Three regions constituting two binding sites were identified encompassing residues 69-79 (I), 87-91 (II), and 118-127 (III). Region III is MalK-specific, whereas residues from regions I and II partly overlap but are not identical to the binding interfaces for interaction with glycerol kinase and lactose permease. These results were fully verified by studying the inhibitory effect of purified EIIA(Glc) variants carrying mutations at positions representative of each of th...Continue Reading

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D WorthylakeS J Remington
Feb 13, 2001·Current Opinion in Biotechnology·U ReinekeJ Schneider-Mergener
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·J ChenA L Davidson
Apr 30, 2003·Protein Science : a Publication of the Protein Society·Guangshun WangAlan Peterkofsky
Jun 11, 2004·Annual Review of Biochemistry·Amy L Davidson, Jue Chen
Jun 14, 2005·Analytical Biochemistry·Armin A WeiserRudolf Volkmer-Engert

❮ Previous
Next ❯

Citations

Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Kai HilperRobert E W Hancock
Mar 25, 2008·Current Opinion in Microbiology·Josef Deutscher
Dec 22, 2007·Research in Microbiology·Vicente MonederoJosef Deutscher
Jul 4, 2013·The Journal of Biological Chemistry·Huan Bao, Franck Duong
Jul 25, 2018·Journal of Bacteriology·Kambiz Morabbi Heravi, Josef Altenbuchner
May 14, 2009·Chembiochem : a European Journal of Chemical Biology·Rudolf Volkmer
Sep 29, 2019·Research in Microbiology·Rebecca MächtelCédric Orelle
Sep 1, 2010·EcoSal Plus·Amy L Davidson, Frances Joan D Alvarez

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.