Topography of the yeast ATP synthase F0 sector by using cysteine substitution mutants. Cross-linkings between subunits 4, 6, and f

Biochemistry
C SpannagelJean Velours

Abstract

The arrangement of the N-terminal part of subunit 4 (subunit b) has been studied by the use of mutants containing cysteine residues in a loop connecting the two N-terminal postulated membrane-spanning segments. Labelling of the mutated subunit 4 by the fluorescent probe N-(7-(dimethylamino)-4-methyl-3-coumarinyl)maleimide revealed that the sulfhydryl groups were modified upon incubation of intact mitochondria. In addition, the nonpermeant sulfhydryl reagent 4-acetamido-4'-maleimidylstilbene-2,2'-disulfonic acid prevented the 3-(N-maleimidylpropionyl)biocytin labeling of subunit 4D54C, thus showing a location of this residue in the intermembrane space. Cross-linking experiments revealed the proximity of subunits 4 and f. In addition a disulfide bridge between subunit 4D54C and subunit 6 was evidenced, thus demonstrating near-neighbor relationships of the two subunits and a location of the N-terminal part of the mitochondrially-encoded subunit 6 in the intermembrane space.

References

Jan 1, 1988·Physiological Reviews·A E Senior
Jan 4, 1988·European Journal of Biochemistry·J VeloursB Guérin
Jul 1, 1968·European Journal of Biochemistry·M Somlo
Aug 23, 1996·The Journal of Biological Chemistry·G ArselinJ Velours
Aug 23, 1996·The Journal of Biological Chemistry·G I BelogrudovY Hatefi

❮ Previous
Next ❯

Citations

Jan 20, 1999·Biochimie·J VeloursN Camougrand
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·Y Saint-GeorgesG Dujardin
Jun 6, 2012·The International Journal of Biochemistry & Cell Biology·Johan HabersetzerPatrick Paumard
Nov 29, 2011·Journal of Structural Biology·Marie-France GiraudAlain Dautant
Feb 22, 2001·Chemistry & Biology·A R SalomonC Khosla
Dec 13, 2003·Biochimica Et Biophysica Acta·Andrew N StephensRodney J Devenish
Jun 20, 1998·The Journal of Biological Chemistry·F I Valiyaveetil, R H Fillingame
Sep 10, 2004·The Journal of Biological Chemistry·Claire LemaireNathalie Bonnefoy

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.