Topological analysis of the ATP-gated ionotropic [correction of ionotrophic] P2X2 receptor subunit

FEBS Letters
G E TorresMark M Voigt

Abstract

We investigated the transmembrane topology of the P2X2 receptor subunit expressed in HEK 293 cells. Initial studies using two P2X subunits expressed in tandem indicated that the amino- and carboxy-termini are on the same side of the membrane. Immunofluorescence studies showed the cytoplasmic orientation of the amino- and carboxy-termini. Finally, N-glycosylation scanning mutagenesis revealed that reporter sites inserted into the central loop, but not those in the amino- or carboxy-terminal regions, were glycosylated, thus suggesting an extracellular placement for that domain. Our results support a two-transmembrane arrangement for P2X receptors with intracellular amino- and carboxy-termini.

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Citations

May 2, 2006·Pflügers Archiv : European journal of physiology·Joel R GeverAnthony P D W Ford
May 19, 2006·Pflügers Archiv : European journal of physiology·Terrance M EganZhiyuan Li
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Mar 21, 2002·FEBS Letters·Martin PaukertStefan Gründer
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Dec 19, 2002·Molecular Pharmacology·Annette NickeGünther Schmalzing
Jul 31, 1999·The Journal of Biological Chemistry·G E TorresM M Voigt
Mar 27, 2001·The Journal of Biological Chemistry·M PaukertS Gründer
Apr 21, 2001·The Journal of Biological Chemistry·M KimA Surprenant
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