Topological and functional analysis of the rat liver carnitine palmitoyltransferase 1 expressed in Saccharomyces cerevisiae

FEBS Letters
Carina Prip-BuusJ Girard

Abstract

The rat liver carnitine palmitoyltransferase 1 (L-CPT 1) expressed in Saccharomyces cerevisiae was correctly inserted into the outer mitochondrial membrane and shared the same folded conformation as the native enzyme found in rat liver mitochondria. Comparison of the biochemical properties of the yeast-expressed L-CPT 1 with those of the native protein revealed the same detergent lability and similar sensitivity to malonyl-CoA inhibition and affinity for carnitine. Normal Michaelis-Menten kinetics towards palmitoyl-CoA were observed when careful experimental conditions were used for the CPT assay. Thus, the expression in S. cerevisiae is a valid model to study the structure-function relationships of L-CPT 1.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F TaroniS DiDonato
May 1, 1989·Diabetes/metabolism Reviews·J D McGarryD W Foster
Dec 1, 1995·Journal of Bacteriology·T A BrownB L Trumpower
Apr 28, 1995·The Journal of Biological Chemistry·F TessonA Parini
Jan 1, 1995·Antonie van Leeuwenhoek·W VisserJ P van Dijken
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·C H BrittonJ D McGarry
Oct 1, 1994·Yeast·A Horvath, H Riezman
Jan 1, 1996·Methods in Enzymology·D PomponP Urban
Feb 15, 1997·European Journal of Biochemistry·J D McGarry, N F Brown
May 1, 1997·The Biochemical Journal·F FraserV A Zammit

❮ Previous
Next ❯

Citations

Feb 5, 2003·Biochemical and Biophysical Research Communications·Jia DaiGebre Woldegiorgis
Jun 17, 2000·Biochimica Et Biophysica Acta·J Kerner, C Hoppel
Apr 21, 2011·Journal of Medicinal Chemistry·Simona M CeccarelliArduino Arduini
May 12, 2009·Journal of Lipid Research·David SebastiánGuillermina Asins
May 12, 2009·Cellular and Molecular Life Sciences : CMLS·Arne C RuferMichael Hennig
Sep 8, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kang-Le LuWen-Bin Liu
Jan 31, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Beatrijs BarteldsFeike R van der Leij
Jul 26, 2007·The Journal of Biological Chemistry·Audrey FayeCarina Prip-Buus
Nov 28, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Abdelhak MansouriNúria Morral
Aug 11, 2016·American Journal of Physiology. Endocrinology and Metabolism·Eliska VavrovaCarina Prip-Buus
Feb 26, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carole HéniqueIsabelle Cohen
Oct 1, 2003·The Journal of Biological Chemistry·Stéphanie GobinCarina Prip-Buus
Mar 11, 2004·European Journal of Biochemistry·Jason M GoodingE David Saggerson
Oct 26, 2006·American Journal of Physiology. Endocrinology and Metabolism·David SebastiánFausto G Hegardt
Jan 15, 2008·The Journal of Biological Chemistry·Adriana Y SierraNúria Casals
Jul 19, 2018·Scientific Reports·Deepti RamachandranAbdelhak Mansouri
Apr 4, 2021·The Journal of Biological Chemistry·Xiao ZhangJia Zeng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.