Topologically Associating Domains: An invariant framework or a dynamic scaffold?

Caelin Cubeñas-Potts, Victor G Corces


Metazoan genomes are organized into regions of topologically associating domains (TADs). TADs are demarcated by border elements, which are enriched for active genes and high occupancy architectural protein binding sites. We recently demonstrated that 3D chromatin architecture is dynamic in response to heat shock, a physiological stress that downregulates transcription and causes a global redistribution of architectural proteins. We utilized a quantitative measure of border strength after heat shock, transcriptional inhibition, and architectural protein knockdown to demonstrate that changes in both transcription and architectural protein occupancy contribute to heat shock-induced TAD dynamics. Notably, architectural proteins appear to play a more important role in altering 3D chromatin architecture. Here, we discuss the implications of our findings on previous studies evaluating the dynamics of TAD structure during cellular differentiation. We propose that the subset of variable TADs observed after differentiation are representative of cell-type specific gene expression and are biologically significant.


Sep 8, 2012·Genome Research·Hao WangJohn A Stamatoyannopoulos
May 28, 2013·Cell·Jennifer E Phillips-CreminsVictor G Corces
Nov 5, 2013·The EMBO Journal·Sevil SofuevaSuzana Hadjur
Dec 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jessica ZuinKerstin S Wendt
Jun 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Carlos Gómez-MarínJosé Luis Gómez-Skarmeta


Jan 7, 2017·Nucleus·A Rasim BarutcuAnthony N Imbalzano
Oct 5, 2017·Nucleic Acids Research·Caitlin HultKerry Bloom
Jun 25, 2016·Genes & Development·Annette Denker, Wouter de Laat
Sep 2, 2017·Cellular & Molecular Biology Letters·Sergey V Razin, Sergey V Ulianov
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Oct 20, 2020·Nucleic Acids Research·Tiantian Ye, Wenxiu Ma
Nov 19, 2020·Trends in Genetics : TIG·Ittai E Eres, Yoav Gilad
May 20, 2021·Journal of Experimental & Clinical Cancer Research : CR·Guo-Hua LiJian Qu

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