PMID: 9169604May 1, 1997Paper

Topology of carnitine palmitoyltransferase I in the mitochondrial outer membrane

The Biochemical Journal
F FraserV A Zammit

Abstract

The topology of carnitine palmitoyltransferase I (CPT I) in the outer membrane of rat liver mitochondria was studied using several approaches. 1. The accessibility of the active site and malonyl-CoA-binding site of the enzyme from the cytosolic aspect of the membrane was investigated using preparations of octanoyl-CoA and malonyl-CoA immobilized on to agarose beads to render them impermeant through the outer membrane. Both immobilized ligands were fully able to interact effectively with CPT I. 2. The effects of proteinase K and trypsin on the activity and malonyl-CoA sensitivity of CPT I were studied using preparations of mitochondria that were either intact or had their outer membranes ruptured by hypo-osmotic swelling (OMRM). Proteinase K had a marked but similar effect on CPT I activity irrespective of whether only the cytosolic or both sides of the membrane were exposed to it. However, it affected sensitivity more rapidly in OMRM. By contrast, trypsin only reduced CPT I activity when incubated with OMRM. The sensitivity of the residual CPT I activity was unaffected by trypsin. 3. The proteolytic fragments generated by these treatments were studied by Western blotting using three anti-peptide antibodies raised against linear...Continue Reading

Citations

Dec 29, 2015·Progress in Lipid Research·Núria CasalsDolors Serra
Jul 6, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Evridiki BoukouvalaGrigorios Krey
Apr 8, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qiaoli JiJiye Zhang
Sep 4, 2008·Proteomics·Anne M DistlerCharles L Hoppel
Nov 4, 2006·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Izaäk Frederik KoddeJan Willem de Jong
Feb 5, 2003·Biochemical and Biophysical Research Communications·Jia DaiGebre Woldegiorgis
Sep 26, 2000·Molecular Genetics and Metabolism·F R van der LeijB Bartelds
Feb 24, 2015·Frontiers in Cellular Neuroscience·Esther Gratacòs-BatlleDavid Soto
Jul 5, 2008·Annual Review of Nutrition·David Saggerson
Apr 19, 2008·IUBMB Life·Victor A Zammit
Dec 4, 2004·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Naoshi Yamazaki
Oct 13, 2005·American Journal of Physiology. Endocrinology and Metabolism·Veronic BezaireLawrence L Spriet
Jun 15, 2007·American Journal of Physiology. Endocrinology and Metabolism·Erik van Lunteren, Michelle Moyer
Jul 26, 2007·The Journal of Biological Chemistry·Audrey FayeCarina Prip-Buus
Oct 15, 2010·Free Radical Research·Janos KernerCharles L Hoppel
May 31, 2011·The Journal of Biological Chemistry·Kwangwon LeeCharles L Hoppel
Jan 5, 2020·Endocrinology·Isabel R Schlaepfer, Molishree Joshi
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Abu-ElheigaS J Wakil
Jul 16, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Minéia WeberDolors Serra
Oct 1, 2003·The Journal of Biological Chemistry·Stéphanie GobinCarina Prip-Buus
Oct 13, 2011·The Journal of Biological Chemistry·Jampani N RaoTobias S Ulmer
Apr 26, 2012·Human & Experimental Toxicology·W WangK Zhang
Aug 24, 2018·Frontiers in Molecular Neuroscience·Esther Gratacòs-BatlleDavid Soto

❮ Previous
Next ❯