Topology of the secondary structure elements of ribosomal protein L7/L12 from E. coli in solution

FEBS Letters
E V BocharovA S Arseniev

Abstract

Topology of the secondary structure elements of ribosomal protein L7/L12 has been studied. The sequential assignment was obtained for main and side chain resonances. This allows the overall secondary structure to be described. The results of high resolution NMR studies show that dimer of the ribosomal protein L7/L12 from Escherichia coli has a parallel (head-to-head) orientation of subunits, and N-terminal domain (NTD, residues Ser1-Ser33) has no contacts with the C-terminal domain (CTD, residues Lys51-Lys120). The NMR data for CTD are in line with crystallographic structure. The flexible interdomain (hinge) region (residues Ala34-Glu50) has an unordered structure, the Pro44 forming both cis and trans peptide bonds. Due to the conformational exchange the intensities of the peaks from the NTD are low. The conformation of the NTD, which is responsible for the formation of the L7/L12 dimer, is alpha-helical hairpin. the NTD dimer forms an antiparallel four-alpha-helix bundle.

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Citations

Aug 26, 2000·Progress in Biophysics and Molecular Biology·S Al-KaradaghiA Liljas
Oct 23, 1997·Nature Structural Biology·A Liljas, S al-Karadaghi
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·A V OleinikovR R Traut
Aug 15, 1998·Biological Chemistry·S V NikonovM B Garber
Jul 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·John ChristodoulouChristopher M Dobson
Dec 25, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Joanna WillDirk Wolters
May 9, 2000·The Journal of Biological Chemistry·D I Svergun, K H Nierhaus
Jan 1, 2018·Quarterly Reviews of Biophysics·Anders Liljas, Suparna Sanyal
Sep 21, 2019·Scientific Reports·Xiaolin WangJohn Christodoulou
Feb 13, 2004·The Journal of Biological Chemistry·Eduard V BocharovAlexander S Arseniev
Jun 4, 2021·Biochemistry·JianChao YuAlexander Shekhtman

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