TOR and PKA pathways synergize at the level of the Ste11 transcription factor to prevent mating and meiosis in fission yeast.

PloS One
Noelia Valbuena, Sergio Moreno

Abstract

In the fission yeast Schizosaccharomyces pombe, the TOR (target of rapamycin) and PKA (protein kinase A) signaling transduction pathways regulate the expression of genes required for cell growth and sexual differentiation in response to the nutritional environment. Inhibition of Tor2 signaling results in the induction of genes involved in sexual differentiation, and the cells undergo mating and meiosis, even under good nutritional conditions. The same phenotype is observed in mutants in which the PKA pathway is inactive. By contrast, Tor2 overexpression or mutations that hyperactivate PKA signaling impair sexual differentiation, even under poor nutritional conditions. Accordingly, a very important question is to understand the molecular mechanism by which these two pathways coordinately regulate gene expression in response to nutrients. Here we demonstrate that TOR and PKA pathways operate coordinately to negatively regulate sexual differentiation by inhibiting the nuclear accumulation of the Ste11 transcription factor. However, the Tor2 pathway is unable to block the nuclear localization of Ste11 under good nutritional conditions when the PKA pathway is inactive. Using microarray analyses, we found that both pathways inhibit s...Continue Reading

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Citations

Nov 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Folkert J van Werven, Angelika Amon
Oct 22, 2014·Applied Microbiology and Biotechnology·Stefan HennigKai Ostermann
Jan 19, 2012·PloS One·Ana M Matia-GonzalezMiguel A Rodriguez-Gabriel
Jul 4, 2017·Biomolecules·Yoko OtsuboAkira Yamashita
Sep 22, 2017·FEMS Yeast Research·Hokuto Ohtsuka, Hirofumi Aiba
Dec 18, 2013·Molecular and Cellular Biology·Dana LaorRonit Weisman
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Feb 21, 2021·Yeast·Gaurav Barve, Ravi Manjithaya
Aug 11, 2012·Journal of Cell Science·Yoko Otsubo, Masayuki Yamamoto

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Methods Mentioned

BETA
GTPase
FACS
flow cytometry
fluorescence microscopy

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