PMID: 2106365Feb 12, 1990Paper

Torpedo synaptophysin: evolution of a synaptic vesicle protein

Brain Research
D M CowanR H Scheller

Abstract

Synaptophysin is an integral membrane protein of synaptic vesicles found in neurons and endocrine cells. Synaptophysin monomers associate into hexamers forming a large conductance channel. We present an analysis of synaptophysin from the nervous system of the marine ray Torpedo californica. Analysis of cDNA clones reveals a 62% amino acid similarity between the Torpedo and rat sequences. The 4 hydrophobic membrane spanning domains and the glycosylation site are conserved. In contrast, the two intravesicular loops connecting the membrane spanning regions, show varying degrees of sequence conservation, suggesting that portions of these domains may play critical functional roles. The carboxyterminal tail has been proposed to bind calcium and is a major site for tyrosine phosphorylation. The precise sequence of this region has almost completely diverged while the proline-tyrosine rich nature is maintained. Blotting studies reveal the RNA and the protein in nervous system tissues and demonstrate that the molecule copurifies with cholinergic synaptic vesicles.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R RahamimoffN Yakir
Dec 15, 1986·Biochemical Pharmacology·P De Camilli, P Greengard
Nov 25, 1987·Nucleic Acids Research·T C SüdhofR Jahn
Dec 1, 1987·The Journal of Cell Biology·K M BuckleyR B Kelly
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W S TrimbleR H Scheller
Jan 1, 1987·Cold Spring Harbor Symposia on Quantitative Biology·R H Kretsinger
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·R JahnP Greengard
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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Citations

Jun 1, 1991·Cell and Tissue Research·P RedeckerD Grube
Jun 1, 1994·Cellular and Molecular Neurobiology·E L Ochoa
Jan 1, 1990·Molecular Neurobiology·E L OchoaM G McNamee
Oct 1, 1991·Brain Research. Molecular Brain Research·W VolknandtR H Scheller
May 1, 1992·Brain Research. Molecular Brain Research·J L Bixby
Jun 10, 1996·Biochimica Et Biophysica Acta·M Linial, D Parnas
Jan 1, 1995·Neuroscience·W Volknandt
Apr 29, 1998·Brain Research. Molecular Brain Research·B W BernsteinJ R Bamburg
Nov 1, 1990·The Journal of Cell Biology·L Thomas, H Betz
Feb 1, 1992·The Journal of Cell Biology·M K BennettR H Scheller
Mar 1, 1993·Journal of Neurochemistry·W VolknandtH Zimmermann
Jul 1, 1993·Journal of Neurochemistry·R Jahn, T C Südhof
May 1, 1992·Journal of Cellular Biochemistry·A ShalevM Linial
May 13, 2008·Clinical Psychology Review·Jemima Petch, W Kim Halford
Jul 9, 1993·Brain Research·K G MillerR H Scheller
Jun 1, 1991·Neuron·B WendlandR H Scheller
Sep 27, 1996·Neuroscience Letters·B H Sokolowski, A M Cunningham

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