PMID: 2125477Oct 16, 1990Paper

Torsional stress stabilizes extended base unpairing in suppressor sites flanking immunoglobulin heavy chain enhancer

Biochemistry
T Kohwi-Shigematsu, Y Kohwi

Abstract

DNA sequences surrounding the immunoglobulin heavy chain (IgH) enhancer contain negative regulatory elements which are important for the tissue specificity of the enhancer. We have shown that sequences located both 5' and 3' of the enhancer, corresponding to the negative regulatory elements, become stably and uniformly unpaired over an extended length when subjected to torsional stress. These DNA sequences are also included within matrix association regions. The ability of the sequences to assume a stably unpaired conformation was shown by reactivity with chloroacetaldehyde which is specific for unpaired DNA bases, as well as two-dimensional gel electrophoresis of topoisomers. The sequences located 3' of the enhancer induce base unpairing in the direction of the enhancer. This unpaired region progressively expands to include as much as 200 base pairs as the ionic concentration decreases or superhelical density increases. When an ATATAT motif within a negative regulatory element located 3' of the enhancer was mutated, the extensive base-unpairing property was abolished. This base-unpairing property of DNA may be important for negative regulation of gene expression and attachment to the nuclear matrix.

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J C Wang
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D KowalskiM J Eddy
Jun 11, 1987·Nucleic Acids Research·R M Umek, D Kowalski
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y Kohwi, T Kohwi-Shigematsu
Oct 20, 1987·Journal of Molecular Biology·J A McClellan, D M Lilley
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·T Kohwi-ShigematsuY Kohwi
Jan 20, 1987·Journal of Molecular Biology·K M Sullivan, D M Lilley
Jan 31, 1972·Biochemical and Biophysical Research Communications·J R BarrioN J Leonard
Jun 1, 1983·Cell·G Khoury, P Gruss
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Kohwi-ShigematsuH Weintraub
Jan 1, 1983·Cold Spring Harbor Symposia on Quantitative Biology·J C WangK Becherer

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Citations

Sep 6, 2000·Journal of Cellular Biochemistry·N N KhodarevA T Vaughan
Jan 1, 1993·Environmental and Molecular Mutagenesis·J L Schwartz, A T Vaughan
May 7, 1992·Biochimica Et Biophysica Acta·A I MurchieD M Lilley
Feb 19, 2003·Journal of Molecular Biology·Vladimir N PotamanRichard R Sinden
Jun 11, 2009·Quarterly Reviews of Biophysics·Tali E Haran, Udayan Mohanty
Oct 11, 2002·Nature·Dag YasuiTerumi Kohwi-Shigematsu
Apr 18, 2003·Cell Death and Differentiation·Q Y LiuM Sikorska
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H M Darwish, H F DeLuca
Mar 14, 1998·Nucleic Acids Research·Z AvramovaJ L Bennetzen
Jun 26, 2003·Nucleic Acids Research·Yanlin Huang, David Kowalski
Jul 27, 2007·Nucleic Acids Research·Kazuhiko YamasakiKazuaki Harata
Jun 29, 1999·Cold Spring Harbor Symposia on Quantitative Biology·L A FernándezR Grosschedl
Aug 1, 2009·Cancer Cell International·Neill PataniKefah Mokbel
Jan 30, 2008·Genome Biology·Diego OttavianiDenise Sheer
Dec 20, 2012·PloS One·Ellen OrdinarioTerumi Kohwi-Shigematsu
Mar 21, 2006·Zoological Science·Hideki TagashiraKoji Akasaka
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·K WillW Deppert
Jul 8, 1998·Proceedings of the National Academy of Sciences of the United States of America·D MishmarB Kerem
Dec 17, 2008·Women's Health·Jose RussoTerumi Kohwi-Shigematsu
Sep 21, 2011·The Journal of Cell Biology·Michael Y FessingVladimir A Botchkarev
Jan 1, 1991·Critical Reviews in Biochemistry and Molecular Biology·E Palecek
Aug 5, 2003·Journal of Cellular Biochemistry·Ildikó SzilágyiGábor Szabó
Jul 12, 2012·Methods : a Companion to Methods in Enzymology·Terumi Kohwi-ShigematsuShutao Cai
Jul 10, 2012·Seminars in Cancer Biology·Terumi Kohwi-ShigematsuYoshinori Kohwi

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