TosR-Mediated Regulation of Adhesins and Biofilm Formation in Uropathogenic Escherichia coli.

MSphere
Courtney L LuterbachHarry L T Mobley

Abstract

Uropathogenic Escherichia coli strains utilize a variety of adherence factors that assist in colonization of the host urinary tract. TosA (type one secretion A) is a nonfimbrial adhesin that is predominately expressed during murine urinary tract infection (UTI), binds to kidney epithelial cells, and promotes survival during invasive infections. The tosRCBDAEF operon encodes the secretory machinery necessary for TosA localization to the E. coli cell surface, as well as the transcriptional regulator TosR. TosR binds upstream of the tos operon and in a concentration-dependent manner either induces or represses tosA expression. TosR is a member of the PapB family of fimbrial regulators that can participate in cross talk between fimbrial operons. TosR also binds upstream of the pap operon and suppresses PapA production. However, the scope of TosR-mediated cross talk is understudied and may be underestimated. To quantify the global effects of TosR-mediated regulation on the E. coli CFT073 genome, we induced expression of tosR, collected mRNA, and performed high-throughput RNA sequencing (RNA-Seq). These findings show that production of TosR affected the expression of genes involved with adhesins, including P, F1C, and Auf fimbriae, n...Continue Reading

References

Oct 1, 1978·Infection and Immunity·I Ofek, E H Beachey
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K ForsmanB E Uhlin
Jun 1, 1991·Journal of Bacteriology·J A DeMoss, P Y Hsu
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B LundS Normark
Jan 1, 1985·Molecular & General Genetics : MGG·S KnappW Goebel
Aug 1, 1970·Journal of Bacteriology·D C Old, J P Duguid
Nov 1, 1984·Journal of Bacteriology·B NowickiT K Korhonen
Jan 1, 1995·Annual Review of Microbiology·J W CostertonH M Lappin-Scott
Apr 1, 1998·Bioinformatics·T L Bailey, M Gribskov
May 21, 1999·Science·J W CostertonE P Greenberg
Aug 10, 1999·Journal of Bacteriology·H HasmanP Klemm
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Oct 6, 2000·Annual Review of Microbiology·J Hacker, J B Kaper
Oct 16, 2001·Antimicrobial Agents and Chemotherapy·M TrujilloM Laviña
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 24, 2002·International Journal of Antimicrobial Agents·T A OelschlaegerJ Hacker
Sep 11, 2002·Molecular Genetics and Genomics : MGG·M A SchembriP Klemm
Dec 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·R A WelchF R Blattner
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Eliana Rodríguez, Magela Laviña
Jul 10, 2003·Infectious Disease Clinics of North America·Betsy Foxman, Patricia Brown
Oct 27, 2004·Infection and Immunity·Jennifer A SnyderHarry L T Mobley
Oct 22, 2005·Infection and Immunity·Jennifer A SnyderHarry L T Mobley
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·María Eloisa PoeyMagela Laviña
May 18, 2006·Annual Review of Microbiology·Michelle M Barnhart, Matthew R Chapman
Aug 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elzbieta BrzuszkiewiczUlrich Dobrindt

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Methods Mentioned

BETA
RNA-Seq
PCR
Illumina sequencing

Software Mentioned

Multiple Em for Motif Elicitation
MEME
SPARTA

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