Total and covalently closed circular DNA detection in liver tissue of long-term survivors transplanted for HBV-related cirrhosis

Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver
Ilaria LenciMario Angelico

Abstract

Life-long prophylaxis against HBV recurrence is recommended in patients transplanted for HBV-related disease. The risk of HBV reactivation is due to persistence of covalently closed circular (ccc) DNA in hepatocytes. Whether cccDNA persists in livers of long-term transplant survivors who received conventional prophylaxis is unknown. To investigate the presence of intrahepatic total and cccDNA in transplanted patients with no evidence of biochemical markers of HBV recurrence. Intrahepatic total and cccDNA were assessed using sensitive nested and real-time PCR from 44 HBsAg-positive patients (75% male; mean age 55.2+/-8.9 years) who had undetectable serum HBV-DNA at transplant. The mean follow-up after transplant was 88.3 months (range, 18-159). One patient underwent HBV recurrence after transplant and was the only who tested positive for both intrahepatic total HBV-DNA and cccDNA. Of the 43 patients negative for all serological markers of HBV infection, only 2 tested positive for intrahepatic total HBV-DNA, but none for cccDNA. Most patients with undetectable HBV-DNA at transplant, who received conventional HBV prophylaxis, have no evidence of intrahepatic total HBV-DNA and cccDNA. cccDNA should be considered a new additional di...Continue Reading

References

Jan 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S E DaviesR Williams
Apr 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S TodoT E Starzl
Jun 1, 1995·Journal of Hepatology·K IshakR N MacSween
Dec 16, 1993·The New England Journal of Medicine·D SamuelH Bismuth
Mar 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J KöckH J Schlicht
May 1, 1997·Gut·N A Terrault, T L Wright
Jun 6, 1998·Virology·W S MasonA R Jilbert
Aug 8, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J S MarkowitzR W Busuttil
Jun 28, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bruno RocheDidier Samuel
Jul 16, 2003·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Jérôme DumortierOlivier Boillot
Aug 31, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Karsten WursthornJoerg Petersen
Jan 30, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Anna S F Lok, Brian J McMahon

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Citations

Sep 10, 2014·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Francesco TandoiMauro Salizzoni
Jan 5, 2016·Liver International : Official Journal of the International Association for the Study of the Liver·Rajneesh KumarFabien Zoulim
Jul 14, 2010·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Daniel Shouval
Oct 30, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·David Mutimer
May 24, 2011·Journal of Hepatology·Alyson N Fox, Norah A Terrault
Jan 26, 2012·Journal of Hepatology·Alyson N Fox, Norah A Terrault
Jun 9, 2016·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Ilaria LenciMario Angelico
Jul 9, 2016·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Bruno Roche, Didier Samuel
Feb 9, 2018·Expert Review of Anti-infective Therapy·Laura Ambra NicoliniMalgorzata Mikulska
Jun 2, 2020·World Journal of Gastroenterology : WJG·Ilaria LenciLeonardo Baiocchi
Jun 8, 2017·Viruses·Xiaoling LiNingshao Xia

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