PMID: 3145280Nov 1, 1988

Total CA activity in isolated perfused guinea pig lung by 18O-exchange method

Journal of Applied Physiology
S NiokaR E Forster

Abstract

The rate of exchange of 18O between alveolar CO2 and lung water was measured in isolated perfused guinea pig lungs to quantify carbonic anhydrase (CA) activity. The average lung CA activity, with a reaction velocity constant of 5.32 +/- 2.2 s-1, is sufficient to accelerate CO2 reactions in lung water by two orders of magnitude over the uncatalyzed rate at 22 degrees C and a PCO2 of 40 Torr. Three sulfonamide inhibitors of CA with different human erythrocyte membrane permeabilities were used to determine the availability of the enzyme to the perfusate. Ethoxzolamide, the most permeable at 0.1 microM (100 times its inhibition constant, of Ki) inhibited 85% of enzyme activity after exposure of the lung for 3 min and 94% of enzyme activity after 30 min, whereas 1.25 microM (320 times its Ki) acetazolamide (1/165 as permeable) only inhibited CA 28% at 3 min and 75% at 30 min. Benzolamide (less than 1/1,000 as permeable) at 4 microM (1,000 times its Ki) inhibited only approximately 17% of control CA activity by 5 min and 48% by 30 min after the start of perfusion. These data indicate the CA available to pulmonary capillary plasma is approximately 10% of the total lung CA activity, in agreement with published measurements on the homog...Continue Reading

Citations

Aug 9, 2019·International Journal of Molecular Sciences·Rossana Occhipinti, Walter F Boron
Feb 26, 1998·Journal of Applied Physiology·H HellerK D Schuster
Jun 15, 2004·Journal of Applied Physiology·Holger SchulzJoachim Heyder

Related Concepts

Body Water
Carbon Dioxide
Carbonate Dehydratase
Carbonic Anhydrase Inhibitors
Cavia porcellus
Lung
Oxygen Isotopes
Perfusion
Pulmonary Alveolar Structure

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