Total cell-free DNA (beta-globin gene) distribution in maternal plasma at the second trimester: a new prospective for preeclampsia screening

Prenatal Diagnosis
Antonio FarinaTakashi Okai

Abstract

Levels of cell-free foetal DNA (f-DNA) in maternal plasma are higher in those asymptomatic subjects who will eventually develop preeclampsia. f-DNA is, however, informative only for those women bearing a male foetus, by amplification of Y-specific sequences and represents a small fraction of total circulating DNA that can be dosed by using ubiquitous genes as well as beta-globin. In this study, we examined the quantitative distribution of total DNA by amplification of beta-globin gene, in asymptomatic women matched with controls to evaluate its possible role in predicting preeclampsia. Forty-eight low-risk women (8 asymptomatic cases matched for gestational age at the second trimester with 40 controls) were enrolled in the present study. beta-globin concentrations were converted into multiples of the median of the controls (MoM), in order to assess the possible different distribution of beta-globin MoM in cases and controls. MoM values were as follows: controls, 1.00 +/- 0.59; asymptomatic cases, 1.99 +/- 1.95. After Gaussian conversion of data, at a false-positive rate (FPR) of 5%, the detection rate (DR) was 46%. beta-globin (total DNA) levels are higher in those patients who subsequently developed preeclampsia and can potent...Continue Reading

Citations

Jun 13, 2012·International Journal of Hepatology·Franck Chiappini
Jul 16, 2009·Reproductive Biology and Endocrinology : RB&E·Simon GrillOlav Lapaire
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Sep 5, 2006·Prenatal Diagnosis·Antonio FarinaTakashi Okai
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Oct 27, 2021·Expert Review of Molecular Diagnostics·Ana C Palei

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