Total Chemical Synthesis of Modified Histones

Frontiers in Chemistry
Yun-Kun QiBaihui Yan

Abstract

In the post-genome era, epigenetics has received increasing attentions in recent years. The post-translational modifications (PTMs) of four core histones play central roles in epigenetic regulation of eukaryotic genome by either directly altering the biophysical properties of nucleosomes or by recruiting other effector proteins. In order to study the biological functions and structural mechanisms of these histone PTMs, an obligatory step is to prepare a sufficient amount of homogeneously modified histones. This task cannot be fully accomplished either by recombinant technology or enzymatic modification. In this context, synthetic chemists have developed novel protein synthetic tools and state-of-the-art chemical ligation strategies for the preparation of homologous modified histones. In this review, we summarize the recent advances in the preparation of modified histones, focusing on the total chemical synthesis strategies. The importance and potential of synthetic chemistry for the study of histone code will be also discussed.

References

Nov 4, 1994·Science·P E DawsonS B Kent
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·T W MuirP A Cole
Jan 19, 2000·Nature·B D Strahl, C D Allis
Aug 11, 2001·Science·T Jenuwein, C D Allis
Sep 28, 2002·Nature·Helena Santos-RosaTony Kouzarides
Oct 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shu HeDewey G McCafferty
Jan 20, 2005·Molecular and Cellular Biology·Kenneth K LeeJerry L Workman
Mar 17, 2007·Angewandte Chemie·Champak ChatterjeeTom W Muir
Nov 18, 2008·Cell·Tamaki Suganuma, Jerry L Workman
Jan 27, 2009·Chemical Society Reviews·Stephen B H Kent
Mar 9, 2010·Nature Chemical Biology·Champak ChatterjeeTom W Muir
May 4, 2010·Nature·Johanna C ScheuermannJürg Müller
Dec 1, 2010·Chemical Communications : Chem Comm·Xiaohong ZhangChuan-Fa Liu
Jun 8, 2011·Angewandte Chemie·Ge-Min FangLei Liu
Jul 1, 2011·Journal of the American Chemical Society·Ji-Shen ZhengLei Liu
Sep 17, 2011·Angewandte Chemie·Fupeng LiChuan-Fa Liu
Feb 16, 2012·Nature Reviews. Molecular Cell Biology·Lloyd Davis, Jason W Chin
Sep 13, 2012·Angewandte Chemie·Ge-Min FangLei Liu
Sep 18, 2012·Cell·Francesca MattiroliTitia K Sixma
Nov 21, 2012·Journal of the American Chemical Society·Beat FierzTom W Muir
May 7, 2013·Annual Review of Biochemistry·Dinshaw J Patel, Zhanxin Wang
May 25, 2013·Accounts of Chemical Research·Ji-Shen ZhengLei Liu
Jun 14, 2013·Nature·Amélie Fradet-TurcotteDaniel Durocher
Dec 18, 2013·Science·Ping WangSamuel J Danishefsky
Mar 25, 2014·Chemical Reviews·Kathrin Lang, Jason W Chin
Jul 7, 2014·Nature Methods·Uyen T T NguyenTom W Muir
Jul 27, 2014·Methods : a Companion to Methods in Enzymology·Lindsey LongTingting Yao
Sep 10, 2014·Chemical Communications : Chem Comm·Muhammad JbaraAshraf Brik
Oct 21, 2014·Chemical Reviews·Manuel M Müller, Tom W Muir
Dec 6, 2014·Angewandte Chemie·Jia-Xing WangLei Liu
Feb 18, 2015·Chemical Reviews·He HuangYingming Zhao
Mar 19, 2015·Annual Review of Biochemistry·Matthew Holt, Tom Muir
Jun 17, 2015·Angewandte Chemie·Mallikanti SeenaiahAshraf Brik

❮ Previous
Next ❯

Citations

Oct 23, 2018·Protein Science : a Publication of the Protein Society·Stephen B H Kent
Sep 8, 2018·Chembiochem : a European Journal of Chemical Biology·Huasong AiLei Liu
Aug 14, 2020·Chembiochem : a European Journal of Chemical Biology·Jenna N BeyerGeorge M Burslem
Sep 9, 2020·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Zhi-Heng DengJia-Bin Li
Jun 15, 2021·Chemical Science·Xin SuiLei Liu
Mar 1, 2019·ACS Chemical Biology·Catherine A Musselman, Tatiana G Kutateladze
Jul 23, 2020·Cell Chemical Biology·Zhipeng A Wang, Philip A Cole
Nov 26, 2020·Journal of Medicinal Chemistry·Vangelis AgouridasOleg Melnyk
Oct 20, 2018·Journal of the American Chemical Society·Srinivasa Rao AdusumalliVishal Rai
Nov 24, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Huasong AiJia-Bin Li

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
ubiquitination
deubiquitination
protein modification
histone acetylation
acetylation

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Peptide Science : an Official Publication of the European Peptide Society
Suman Kumar MaityAshraf Brik
Biochimica Et Biophysica Acta
Cédric GrauffelAnnick Dejaegere
© 2022 Meta ULC. All rights reserved