Total content of kaurene diterpenes in Annona vepretorum stems via 1 H qNMR: A method for speeding the identification of bioactive extracts

Phytochemical Analysis : PCA
Camila de Souza AraújoJackson Roberto Guedes da Silva Almeida

Abstract

Kaurene diterpenes (KDs) constitute a chemical class often found in the genus Annona with interesting biological activities. To date, chromatographic tools have been mostly used to determine KDs. Quantitative nuclear magnetic resonance (qNMR) has distinguished itself in quantitative estimation of natural products and is an interesting choice to assess total KD contents. To establish a 1 H qNMR method for determining the total KD contents in extracts and fractions obtained from Annona vepretorum stems. Stems were extracted with hexane and methanol, resulting in the hexane extract (HEX-E) and the methanol extract (MeOH-E). The former was partitioned with the acid-base method to obtain the total alkaloid fraction (TA-F) and the neutral dichloromethane fraction (NDM-F). 1 H qNMR measurements were performed on 400 MHz with samples solubilized in deuterated dimethyl sulfoxide. Quantification was carried out using the signals at 4.71 and 4.78 ppm related to hydrogens of the exocyclic double bond of the basic skeleton of KDs and gallic acid as the standard reference. The selectivity, intra- and inter-day precision, reproducibility, limit of detection, limit of quantification, and robustness of the methodology were evaluated. Using the ...Continue Reading

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Citations

Feb 4, 2021·Journal of Analytical Methods in Chemistry·Ping YuXiaoqin Ding

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