Total flavone of Desmodium styracifolium relieved apoptosis and autophagy of COM-induced HK-2 cells by regulating KIM-1 via p38/MAPK pathway

Molecular and Cellular Biochemistry
Haijie XieChun-yu Liu

Abstract

The purpose of the study was to investigate the mechanism of total flavone of Desmodium styracifolium (TFDS) in regulating the formation of urinary calculi. Protein levels of KIM-1, LC3-II, p-p38 were measured by Western blot. The effect of different COM concentrations, different TFDS concentrations, SB203580 (specific inhibitor of p38/MAPK), and overexpression of KIM-1 on cell viability were detected by WST-1 assay. The apoptotic cells and FITC positive cells were detected by flow cytometry. HK-2 cell viability decreased with the increase of COM concentration, and the protein levels of KIM-1, LC3-II, and p-p38 increased with the time. Blocking the p38/MAPK pathway or co-cultured with TFDS inhibited the effects of COM on apoptosis and autophagy of HK-2 cells. In addition, blocking the p38/MAPK pathway inhibited the expression of KIM-1. In COM-induced cells, after treated with SB203580, overexpression of KIM-1 could reverse the protection effect of SB203580 on COM-induced cell damage and the inhibition of SB203580 on COM-induced excessive autophagy, suggesting p38/MAPK regulated KIM-1 to regulate COM-induced cell apoptosis and autophagy. Finally, we proved that TFDS inhibited p38/MAPK pathway. And the protection effect of COM-in...Continue Reading

References

Apr 17, 2007·Phytochemistry·Ming ZhaoChun-Tao Che
Dec 4, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jian-Ming OuyangFeng-Xin Wang
Sep 21, 2012·Kidney International·Vidar O EdvardssonRunolfur Palsson
Oct 23, 2012·Journal of Cellular Physiology·Ai Ing LimJoseph C K Leung
Nov 28, 2013·Canadian Urological Association Journal = Journal De L'Association Des Urologues Du Canada·Shiu-Dong ChungHerng-Ching Lin
Dec 19, 2013·Genes & Cancer·Hari K KoulSweaty Koul
Apr 17, 2016·Molecular and Cellular Biochemistry·Zhiwei Zhang, Cindy X Cai

❮ Previous
Next ❯

Citations

Sep 15, 2020·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Zahide CavdarCaner Cavdar

❮ Previous
Next ❯

Methods Mentioned

BETA
transfections
flow cytometry
Protein Assay

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Rong GouLin Tang
Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Jie BaiPing Liu
International Journal of Clinical and Experimental Medicine
Weiliang DingYongfei Tan
© 2022 Meta ULC. All rights reserved