Total glucosides of Paeonia lactiflora Pall inhibit vascular endothelial growth factor-induced angiogenesis

Journal of Ethnopharmacology
Hui DengJinhua Xu

Abstract

To evaluate the anti-angiogenesis effect of total glucosides of Paeonia lactiflora Pall. In this study, we determined the effect of TGP on the proliferation of human vascular endothelial cells through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and fluorescence-activated cell sorting analysis. A migration assay and a tube formation assay were used to investigate the migration properties and tube formation abilities of human vascular endothelial cells after being treated with TGP. Furthermore, the in vivo anti-angiogenic ability of TGP was determined through a chick chorioallantoic membrane assay. TGP (12.5, 62.5, and 312.5 microg/ml) resulted in a dose-dependent reduction in the proliferation of endothelial cells. This inhibition effect began 6h after treatment and lasted at least 24h. Fluorescence-activated cell sorting analysis data showed an accumulation of cells in the G0/G1 phase of the cell cycle, which exhibited apoptotic features indicative of cell death. The migration properties and tube forming abilities of endothelial cells were dramatically inhibited by the TGP extract. Our results show that TGP can inhibit angiogenesis in vitro and in vivo.

References

Jun 15, 2005·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·L ZhuX-Y Jia
Dec 16, 2005·Nature·Peter Carmeliet
Sep 27, 2006·Journal of Ethnopharmacology·Hong-Mei XuLei Zhang
Apr 26, 2007·Angiogenesis·Carla CostaRaquel Soares
Aug 1, 2007·Nature Clinical Practice. Rheumatology·Dahlia Lainer-Carr, Ernest Brahn
Oct 31, 2007·Nature Clinical Practice. Rheumatology·Zoltán Szekanecz, Alisa E Koch
Apr 5, 2008·The Journal of Pain : Official Journal of the American Pain Society·Xiao-Jun ZhangZhao-Xiang Bian
May 9, 2008·The New England Journal of Medicine·Robert S Kerbel
Jun 26, 2008·Oncology Reports·Ju-Hee LeeSoon-Sun Hong
Jan 20, 2009·Journal of Pharmacological Sciences·Yonggui WuShanyan Lin

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Citations

Jun 19, 2012·International Immunopharmacology·Wei Zhang, Sheng-Ming Dai
Aug 13, 2015·BMC Complementary and Alternative Medicine·Yun Mi LeeJunghyun Kim
Jun 10, 2016·Phytotherapy Research : PTR·Shefton ParkerCharlie Changli Xue

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