Total phenol analysis of weakly supported water using a laccase-based microband biosensor

Analytica Chimica Acta
Alina N SekretaryovaMats Eriksson

Abstract

The monitoring of phenolic compounds in wastewaters in a simple manner is of great importance for environmental control. Here, a novel screen printed laccase-based microband array for in situ, total phenol estimation in wastewaters and for water quality monitoring without additional sample pre-treatment is presented. Numerical simulations using the finite element method were utilized for the characterization of micro-scale graphite electrodes. Anodization followed by covalent modification was used for the electrode functionalization with laccase. The functionalization efficiency and the electrochemical performance in direct and catechol-mediated oxygen reduction were studied at the microband laccase electrodes and compared with macro-scale electrode structures. The reduction of the dimensions of the enzyme biosensor, when used under optimized conditions, led to a significant improvement in its analytical characteristics. The elaborated microsensor showed fast responses towards catechol additions to tap water - a weakly supported medium - characterized by a linear range from 0.2 to 10 μM, a sensitivity of 1.35 ± 0.4 A M(-1) cm(-2) and a dynamic range up to 43 μM. This enhanced laccase-based microsensor was used for water quality...Continue Reading

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Citations

Sep 8, 2017·Biotechnology and Applied Biochemistry·Qian ZhangXuerong Fan
Sep 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ziqi SunLijuan Kou
Mar 14, 2020·International Journal of Biological Macromolecules·Abdelmageed M Othman, Ulla Wollenberger

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