PMID: 9187402Apr 25, 1997Paper

Total plasma homocysteine determination by liquid chromatography before and after methionine loading. Results in cerebrovascular disease

Journal of Chromatography. B, Biomedical Sciences and Applications
M CanditoM Chatel

Abstract

Elevated homocysteine (HCY) levels in tissues and blood are associated with premature occlusive diseases. A number of techniques have been developed to assay HCY, including high-performance liquid chromatography (HPLC) with fluorimetric or electrochemical detection, and radioenzymatic methods. The present study evaluated the adaptation of a liquid chromatographic, ion-exchange technique with postcolumn derivatization using ninhydrin. Fasting and moreover post-methionine load total plasma HCY were assayed in 50 patients three months after a stroke and in 20 age-matched controls. Ion-exchange liquid chromatography was performed on an amino acid analyzer using a modified procedure to improve methionine and HCY separation. HCY values in the fasting state were moderately but significantly increased (P<0.05) in the patients compared to the controls: 10.5+/-3.4 versus 9.3+/-2.3 micromol/l. The difference between the two groups was amplified in post-load HCY results, which were significantly increased (P<0.05) in the patients: 41.6+/-17.8 versus 29.2+/-5.5 micromol/l in controls. The relationship between cerebrovascular disease and impaired HCY metabolism has previously been emphasized by other investigators. Our findings suggest that ...Continue Reading

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Citations

Sep 10, 2013·Analytical and Bioanalytical Chemistry·Pantelis G Rigas
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Véronique DucrosUNKNOWN SFBC Working group on homocysteine. French Society for Clinical Biology
Apr 6, 2004·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·M BecciaM Rasura
May 16, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yanbing Zu
Mar 12, 2004·American Journal of Ophthalmology·Gloria WangRobert N Weinreb
Jan 22, 2010·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Tomas HonzikJiri Zeman
Apr 3, 2016·Current Protocols in Human Genetics·Libin Yuan, J Daniel Sharer
Dec 30, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·I FermoR Paroni
Nov 13, 2004·Analytical Chemistry·Maryline GasthuysJean-Claude Tabet
Aug 5, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Michela TomaiuoloElvira Grandone

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