Total Salvianolic Acid Balances Brain Functional Network Topology in Rat Hippocampi Overexpressing miR-30e

Frontiers in Neuroscience
Qi LiYong Xu

Abstract

We investigated the therapeutic effects and underlying brain functional network topology mechanisms of total salvianolic acid (TSA) treatment for memory dysfunction by using miR-30e overexpression-induced memory deficit in rat hippocampi. Model rats were developed by lentivirus vectors carrying miR-30e into bilateral hippocampus CA1 region through stereo-surgery. Two weeks after surgery, TSA (20 or 10 mg/mL/kg) or saline were administrated for 14 consecutive days. Memory function was assessed by behavioral tests (Y maze and Morris water maze [MWM]); resting-state functional MRI (RS-fMRI); and molecular alterations of BCL-2, UBC9, and Caspase-3 in the hippocampus CA1 region, as detected by immunohistochemistry. Compared to controls, model rats exhibited significantly impaired working and long-term memory in the Y maze and MWM tests (p < 0.01). The brain functional network topology analyzed based on RS-fMRI data demonstrated that miR-30e disturbed the global integration and segregation balance of the brain (p < 0.01), and reduced edge strength between CA1 and the posterior cingulate, temporal lobe, and thalamus (p < 0.05, false discovery rate corrected). At the molecular level, BCL-2 and UBC9 were downregulated, while Caspase-3 w...Continue Reading

References

Aug 23, 2001·Brain Research. Brain Research Reviews·R D'Hooge, P P De Deyn
Jul 11, 2006·European Journal of Pharmacology·Dong Hyun KimJong Hoon Ryu
Oct 13, 2009·NeuroImage·Mikail Rubinov, Olaf Sporns
Nov 13, 2010·World Journal of Gastroenterology : WJG·Ming-Xia WangJing-Yan Han
Dec 20, 2011·Learning & Memory·Annette Jeneson, Larry R Squire
Jul 23, 2013·Brain : a Journal of Neurology·Robert Leech, David J Sharp
Apr 8, 2014·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Hao TangJing-Yan Han
Apr 22, 2014·Current Biology : CB·José de Bourbon-TelesDavid Soto
Jun 24, 2014·Radiology·Frederik BarkhofSerge A R B Rombouts
Jan 24, 2015·Journal of Alzheimer's Disease : JAD·Fabrizio VecchioPaolo Maria Rossini
Mar 1, 2015·Journal of Neuroscience Methods·J D KruschwitzH Walter
May 20, 2015·Journal of Experimental Neuroscience·Junichi KitanakaMotohiko Takemura
Jun 17, 2015·International Journal of Biological Sciences·Luning SunZhi Li
Feb 19, 2016·Scientific Reports·Qi YuanGuangju Ji
Apr 15, 2016·Neuroinformatics·Chao-Gan YanYu-Feng Zang
Apr 27, 2016·Data in Brief·Fatima A NasrallahKai-Hsiang Chuang
Jun 20, 2016·Brain Imaging and Behavior·Lori L Beason-HeldSusan M Resnick
Nov 5, 2016·NeuroImage. Clinical·Djalel-Eddine MeskaldjiDimitri Van De Ville
Jun 10, 2017·Frontiers in Cellular Neuroscience·Allison L McIntoshAndrew Harkin
Jun 5, 2018·Journal of Alzheimer's Disease & Parkinsonism·Salil Sharma, Hui-Chen Lu
Jun 5, 2018·Progress in Neuro-psychopharmacology & Biological Psychiatry·Xiaoyan SunJin-Hui Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection

Software Mentioned

DPABI Image Calculator
GraphPad Prism
Data Processing & Analysis of Brain Imaging ( DPABI )
GraphVar Statistical Toolbox
GraphVar
Cytoplasmic V2
MRIcro

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis