PMID: 7029537Sep 1, 1981Paper

Total synthesis of a RNA molecule with sequence identical to that of Escherichia coli formylmethionine tRNA

Proceedings of the National Academy of Sciences of the United States of America
E OhtsukaM Ikehara

Abstract

A RNA molecule has been synthesized that is identical in sequence to Escherichia coli tRNAfMet except that it lacks the base modifications present in the E. coli tRNA. This was achieved by enzymatic joining of chemically synthesized oligonucleotides with chain lengths of 3-10 which were synthesized by the phosphodiester or phosphotriester method. First, quarter molecules of tRNA were constructed by joining of chemically synthesized fragments with RNA ligase. The 5'-quarter molecule (bases 1-20) served as an acceptor in joining reactions with the 3',5'-bisphosphorylated donor molecule (bases 21-34). The 5'-half molecule thus obtained was treated with phosphatase and joined to the 3'-half molecule which was prepared by ligation of the other quarter molecules (bases 35-60, acceptor; bases 61-77, donor) followed by 5'-phosphorylation with polynucleotide kinase. The synthetic tRNA was characterized by oligonucleotide pattern and was partially active in aminoacylation with E. coli methionyl-tRNA synthetase.

References

Feb 16, 1979·Science·H G Khorana
Dec 1, 1977·European Journal of Biochemistry·E OhtsukaA F Markham
Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·G C WalkerR I Gumport
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Citations

Aug 30, 2008·Science in China. Series C, Life Sciences·H JiangD Wang
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K K OgilvieR J Cedergren
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J R Sampson, O C Uhlenbeck
Nov 11, 1982·Nucleic Acids Research·E OhtsukaD Söll
Oct 11, 1992·Nucleic Acids Research·D GasparuttoR Téoule
Apr 11, 1992·Nucleic Acids Research·R IwaseK Miura
Apr 15, 1988·Biochemical and Biophysical Research Communications·Z J Lesnikowski
Oct 15, 1990·Biochemical and Biophysical Research Communications·E UchidaT Hayakawa
Dec 7, 2010·Chembiochem : a European Journal of Chemical Biology·Eduardo Paredes, Subha R Das
Aug 4, 1983·Nature·B Griffin, T Lindahl

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