Toward a better understanding of the mechanisms of symbiosis: a comprehensive proteome map of a nascent insect symbiont

PeerJ
François RenozThierry Hance

Abstract

Symbiotic bacteria are common in insects and can affect various aspects of their hosts' biology. Although the effects of insect symbionts have been clarified for various insect symbiosis models, due to the difficulty of cultivating them in vitro, there is still limited knowledge available on the molecular features that drive symbiosis. Serratia symbiotica is one of the most common symbionts found in aphids. The recent findings of free-living strains that are considered as nascent partners of aphids provide the opportunity to examine the molecular mechanisms that a symbiont can deploy at the early stages of the symbiosis (i.e., symbiotic factors). In this work, a proteomic approach was used to establish a comprehensive proteome map of the free-living S. symbiotica strain CWBI-2.3T. Most of the 720 proteins identified are related to housekeeping or primary metabolism. Of these, 76 were identified as candidate proteins possibly promoting host colonization. Our results provide strong evidence that S. symbiotica CWBI-2.3T is well-armed for invading insect host tissues, and suggest that certain molecular features usually harbored by pathogenic bacteria are no longer present. This comprehensive proteome map provides a series of candid...Continue Reading

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Citations

Jun 26, 2020·Molecular Ecology·Ross Cunning, Andrew C Baker
May 7, 2019·Frontiers in Physiology·Marisa SkaljacAndreas Vilcinskas
May 1, 2019·Frontiers in Microbiology·Inès PonsThierry Hance
Dec 6, 2020·Applied and Environmental Microbiology·Katherine M ElstonJeffrey E Barrick
Jan 19, 2019·Ecotoxicology and Environmental Safety·Rui CompanyAmparo Torreblanca

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Datasets Mentioned

BETA
CCES01000000
CCES00000000

Methods Mentioned

BETA
genetic modification
reverse-phase chromatography
motility assay

Software Mentioned

ProteinPilot
ABSciex
PSORTb

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