Toward a full characterization of native starch: separation and detection by size-exclusion chromatography

Journal of Chromatography. a
Ngoc-Ly HoangPatrice Castignolles

Abstract

The structure of starch molecules is relevant to nutrition and industrial applications. Size-exclusion chromatography (SEC, also known as GPC) of native starch generally suffers non-satisfactory repeatability and reproducibility of the dissolution and separation. This work combines two polar organic solvents: dimethylsulfoxide for complete dissolution and dimethylacetamide to limit shear degradation. The separation is as repeatable as that of polystyrene standards performing dissolution and separation at 80 degrees C. Successful covalent-labeling on the glucose unit is claimed to be published here for the first time in non-degradative conditions and allows the use of UV detector with significantly higher sensitivity than with a refractometer.

References

Nov 17, 2001·Biomacromolecules·S RadostaW Vorwerg
Feb 26, 2004·Journal of Biochemical and Biophysical Methods·Leopold K KostanskiArchie E Hamielec
Jul 12, 2005·Biomacromolecules·Jeffrey V CastroMelissa A Fitzgerald
Aug 23, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Werner Praznik, Anton Huber
Mar 15, 2006·Biomacromolecules·Rachelle M WardMelissa A Fitzgerald
Feb 26, 2008·Biomacromolecules·Javier M HernándezRobert G Gilbert
Apr 2, 2008·Journal of Chromatography. a·Marianne GaborieauPatrice Castignolles

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Citations

Nov 26, 2010·Analytical and Bioanalytical Chemistry·Robert G Gilbert
Dec 18, 2008·Macromolecular Bioscience·Sarah SchmitzMarianne Gaborieau
Oct 21, 2010·Journal of Separation Science·Francisco Vilaplana, Robert G Gilbert
Oct 26, 2010·Journal of Separation Science·Patrice Castignolles, Marianne Gaborieau
Jul 15, 2020·Biomolecules·Henrike BrustJoerg Fettke
May 11, 2011·Journal of Agricultural and Food Chemistry·Amy Hui-Mei LinTing-Jang Lu

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