Toward evidence-based severity assessment in rat models with repeated seizures: I. Electrical kindling

Epilepsia
Christina MöllerH Potschka

Abstract

Rodent epilepsy models can significantly contribute to our understanding of pathophysiological mechanisms and to validation of biomarker and target candidates. Evidence-based severity assessment is a presupposition for the ethical evaluation of animal experimentation allowances as well as for the development of efficacious refinement concepts. Aiming to improve our understanding of the impact of experimental procedures and repeated seizures, we have completed a comprehensive behavioral and biochemical analysis assessing various parameters that can inform about the influence of an electrical kindling paradigm on well-being in rats. Thereby, we have focused on the immediate effects of phases with focal and generalized seizures with behavioral testing during kindling acquisition. Electrode implantation exerted mild effects on anxiety-associated behavior and reduced serum corticosterone at 3 weeks, but not 7 weeks, following surgery. Analysis in kindled rats excluded any relevant impact of focal seizures on behavioral and biochemical parameters. Assessment in rats with generalized seizures revealed an impact on nest complexity scores, nest soiling, and selected parameters in paradigms evaluating anxiety-associated behavior. Moreove...Continue Reading

References

Aug 1, 1996·Neuroscience·V HelferA Depaulis
Jan 12, 2005·The Journal of Endocrinology·S A CavigelliM K McClintock
Mar 31, 2005·Bioinformatics·Aedín C CulhaneDesmond G Higgins
Mar 21, 2007·Epilepsy & Behavior : E&B·Andréy MazaratiRaman Sankar
May 22, 2007·Neurobiology of Disease·Anton PekcecHeidrun Potschka
Jul 21, 2007·Laboratory Animals·M LepschyR Palme
Jul 26, 2011·Physiology & Behavior·Earl Thomas, Deborah J Gunton
Sep 25, 2012·Neuron·Colin BlakemoreArthur Sussman
Mar 16, 2013·Histochemistry and Cell Biology·Esther AsanKlaus-Peter Lesch
Oct 22, 2014·Lab Animal·Henriëtte J BoutEdgar D Karssing
Nov 16, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fabrizio ScorranoAntonio Armario
Feb 25, 2015·Nature Neuroscience·Brian P Grone, Scott C Baraban
Apr 1, 2015·European Journal of Pharmacology·Melanie L Graham, Mark J Prescott
Apr 15, 2015·Annals of Neurology·Christel BeckerChristophe Bernard
Sep 15, 2015·Cambridge Quarterly of Healthcare Ethics : CQ : the International Journal of Healthcare Ethics Committees·Hope R Ferdowsian, John P Gluck
Sep 17, 2015·Journal of Neuroscience Methods·Katie LidsterMark J Prescott
Mar 15, 2016·General and Comparative Endocrinology·Sumeet MinhasRussell D Romeo
Jul 26, 2016·Frontiers in Behavioral Neuroscience·Shang-Der ChenFu-Zen Shaw
Nov 24, 2017·Seizure : the Journal of the British Epilepsy Association·Lorenz B DehnTheodor W May
Dec 11, 2017·Epilepsy & Behavior : E&B·Mariusz S WigluszWiesław J Cubała

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Citations

Nov 14, 2019·Laboratory Animals·Lydia M KeublerAndré Bleich
Mar 25, 2020·Annals of the New York Academy of Sciences·Simone KumstelDietmar Zechner
Jul 25, 2019·Laboratory Animals·Kerstin SchwabeHeidrun Potschka
Nov 30, 2019·Animals : an Open Access Journal From MDPI·Paulin JirkofLars Lewejohann
Jun 4, 2020·Scientific Reports·Simeon O A HelgersKerstin Schwabe
Nov 15, 2020·Scientific Reports·Guanglin TangDietmar Zechner

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