PMID: 8985404Jan 1, 1997Paper

Toward highly efficient cell-type-specific gene transfer with retroviral vectors displaying single-chain antibodies

Journal of Virology
T H Chu, R Dornburg

Abstract

Recently, we constructed retroviral vector particles derived from spleen necrosis virus (SNV) that display a single-chain antibody (scA) on the viral surface. By transient transfection protocols, we showed that such particles are competent for infection and cell type specific. Efficient infection was dependent on the presence of wild-type envelope, although wild-type SNV was not infectious on target cells (T.-H. T. Chu and R. Dornburg, J. Virol. 69:2659-2663, 1995; T.-H. T. Chu, I. Martinez, W. C. Sheay, and R. Dornburg, Gene Ther. 1:292-299, 1994). In this study, stable packaging lines were constructed and detailed biological and biochemical studies were performed. Chimeric scA-envelope fusion proteins were expressed as efficiently as wild-type envelope and were stable over a period of at least 6 h. Only a fully functional wild-type envelope could act as a helper for efficient virus penetration. The ratio of wild-type envelope protein to chimeric envelope protein appears to determine the efficiency of infection. Virus titers of targeting vectors obtained from stable packaging lines were as high as 10(4) CFU/ml. A 25-fold concentration of vector virus stocks resulted in a 200-fold increase in virus titers (up to 10(6) CFU/ml). ...Continue Reading

References

Jun 1, 1984·Molecular and Cellular Biology·S Kawai, M Nishizawa
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·N V SomiaI M Verma
Jul 1, 1995·Journal of Virology·I Martinez, R Dornburg
Jan 1, 1993·Annual Review of Biochemistry·R A Morgan, W F Anderson
Mar 11, 1993·Nucleic Acids Research·S J RussellG Winter
Jan 1, 1993·Annual Review of Immunology·D Cournoyer, C T Caskey
Sep 1, 1996·Journal of Virology·I Martinez, R Dornburg

❮ Previous
Next ❯

Citations

Jun 1, 1997·Biological Chemistry·S SpadaA Plückthun
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y ZhaoW F Anderson
Oct 24, 2000·Journal of Virology·S SharmaT Friedmann
Mar 9, 2002·Journal of Virology·Timothy J Gollan, Michael R Green
Jul 20, 2007·Journal of Virology·Pankaj KumarLorraine M Albritton
Jan 5, 2001·Expert Opinion on Investigational Drugs·D S Strayer
Oct 30, 1998·Human Gene Therapy·M PelegrinM Piechaczyk
Jun 14, 2003·Human Gene Therapy·Chien-Kuo TaiNoriyuki Kasahara
May 27, 2005·Acta Pharmacologica Sinica·Laura SanzLuis Alvarez-Vallina
Aug 17, 2000·Biochemical and Biophysical Research Communications·D G MottersheadC Oker-Blom
May 18, 2000·Reviews in Medical Virology·G PalùM Pizzato
May 25, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Claudia M Kowolik, Jiing-Kuan Yee
Sep 26, 1997·Molecular Medicine Today·M MarinM Piechaczyk
Oct 1, 2003·Virology·Zahida ParveenRalph Dornburg
Dec 22, 2010·ACS Chemical Biology·Johannes H Urban, Christoph A Merten

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.