Toward the measurement of multiple fluorescence lifetimes in flow cytometry: maximizing multi-harmonic content from cells and microspheres

Journal of Biophotonics
Patrick JenkinsJessica P Houston

Abstract

Flow cytometry is a powerful means for in vitro cellular analyses where multi-fluorescence and multi-angle light scattering can indicate unique biochemical or morphological features of single cells. Yet, to date, flow cytometry systems have lacked the ability to capture complex fluorescence dynamics due to the transient nature of flowing cells. In this contribution we introduce a simple approach for measuring multiple fluorescence lifetimes from a single cytometric event. We leverage square wave modulation, Fourier analysis, and high frequency digitization and show the ability to resolve more than one fluorescence lifetime from fluorescently-labelled cells and microspheres. Illustration of a flow cytometer capable of capturing multiple fluorescence lifetime measurements; creating potential for multi-parametric, time-resolved signals to be captured for every color channel.

References

Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B L SailerH A Crissman
Dec 16, 1997·Biophysical Chemistry·H MalakJ R Lakowicz
Feb 22, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·H Helen CuiHarry A Crissman
Aug 3, 2004·Nature Reviews. Immunology·Stephen P PerfettoMario Roederer
May 12, 2005·Physiological Measurement·Martin HammerEkkehart Königsdörffer
Jul 25, 2006·Nature Medicine·Pratip K ChattopadhyayMario Roederer
Feb 3, 2007·Analytical Chemistry·Noël BoensAtsushi Miura
Jan 13, 2009·Immunology·Pratip K ChattopadhyayMario Roederer
Jul 28, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jessica P HoustonJames P Freyer
Apr 11, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Pratip K ChattopadhyayMario Roederer
Nov 10, 2012·Nature Protocols·Stephen P PerfettoMario Roederer
Feb 8, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Richard NguyenMario Roederer
Mar 29, 2014·Electrophoresis·Wenyan LiJessica P Houston

❮ Previous
Next ❯

Citations

Jul 15, 2016·Optics Express·Ruofan CaoJessica P Houston
Oct 29, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Faisal AlturkistanyJessica P Houston
Aug 14, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Kapil NichaniJessica P Houston
Aug 14, 2021·Frontiers of Physics·Jesus SambranoJessica P Houston

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved