PMID: 8581386Oct 1, 1995Paper

Toward the production of bispecific antibody fragments for clinical applications

Journal of Hematotherapy
P CarterZ Zhu

Abstract

The clinical potential of bispecific antibodies (BsAb) has been hindered by the difficulty of obtaining clinical grade material, together with the immunogenicity of rodent-derived BsAb in patients. The supply issue is being directly addressed by recombinant methods for BsAb fragment production reviewed here. The immunogenicity issue will likely be overcome by the use of humanized or human antibodies. Currently, three technologies appear suitable for the production of BsAb fragments for clinical applications: BsF(ab')2 assembled from Fab' fragments expressed in Escherichia coli, BsF(ab')2 assembled using leucine zippers, and diabodies.

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Citations

Apr 1, 1997·Protein Science : a Publication of the Protein Society·Z ZhuP Carter
Feb 27, 2001·Journal of Immunological Methods·P Carter
Aug 8, 2002·Journal of Immunological Methods·Dan LuZhenping Zhu
Sep 13, 2003·Journal of Immunological Methods·Dan LuZhenping Zhu
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Aug 1, 1997·Current Opinion in Biotechnology·P Carter, A M Merchant
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May 27, 2005·Acta Pharmacologica Sinica·Jonathan S Marvin, Zhenping Zhu
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Aug 4, 1998·International Journal of Cancer. Journal International Du Cancer·S M KipriyanovM Little
Aug 10, 2010·Acta Pharmacologica Sinica·Jing Li, Zhenping Zhu
Feb 17, 2006·The Journal of Biological Chemistry·Juqun ShenZhenping Zhu
Dec 13, 2017·The Journal of Biological Chemistry·Preeti Sharma, David M Kranz
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Oct 8, 1999·Journal of Molecular Biology·S M KipriyanovM Little
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