Towards a clinical application of freeze-dried squalene-based nanomedicines

Journal of Drug Targeting
Marie RouquetteSinda Lepetre-Mouelhi

Abstract

Squalene-adenosine (SQAd) nanoparticles (NPs) were found to display promising pharmacological activity similar to many other nanomedicines, but their long-term stability was still limited, and their preparation required specific know-how and material. These drawbacks represented important restrictions for their potential use in the clinic. Freeze-drying nanoparticles is commonly presented as a solution to allow colloidal stability, but this process needs to be adapted to each nanoformulation. Hence, we aimed at developing a specific protocol for freeze-drying SQAd NPs while preserving their structural features. NPs were lyophilised, resuspended and analysed by dynamic light scattering, atomic force microscopy and small-angle scattering. Among four different cryoprotectants, trehalose was found to be the most efficient in preserving NPs physico-chemical characteristics. Interestingly, we identified residual ethanol in NP suspensions as a key parameter which could severely affect the freeze-drying outcome, leading to NPs aggregation. Long-term stability was also assessed. No significant change in size distribution or zeta potential could be detected after three-month storage at 4 °C. Finally, freeze-dried NPs innocuity was checke...Continue Reading

References

Sep 9, 1999·International Journal of Pharmaceutics·C Schwarz, W Mehnert
Nov 23, 2006·Advanced Drug Delivery Reviews·Wassim AbdelwahedHatem Fessi
May 29, 2009·Journal of Pharmaceutical Sciences·Min Kyung LeeJonghwi Lee
Nov 7, 2009·Journal of Pharmaceutical Sciences·Per WessmanDenny Mahlin
Feb 26, 2010·AAPS PharmSciTech·Praveen V DatePadma V Devarajan
Nov 26, 2010·Chemical Society Reviews·Tanya C Boorman, Igor Larrosa
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Heidi D AndersenPeter Westh
Mar 1, 2011·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·L G SouzaR N Marreto
Jul 23, 2011·Genes & Cancer·Zhaohui FengRui Wu
Mar 29, 2013·Nature Reviews. Drug Discovery·Jiang-Fan ChenBertil B Fredholm
Jul 28, 2013·International Journal of Pharmaceutics·Antonio RampinoAttilio Cesàro
Oct 9, 2013·Pharmaceutical Development and Technology·Slavomira DoktorovovaEliana B Souto
Jan 5, 2014·Advanced Drug Delivery Reviews·Elise LepeltierPatrick Couvreur
Feb 14, 2014·Journal of Applied Toxicology : JAT·Saura C SahuRobert L Sprando
Nov 25, 2014·Nature Nanotechnology·Alice GaudinPatrick Couvreur
Apr 16, 2015·Soft Matter·Debasish SahaOlivier Spalla
Jun 19, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Alice GaudinPatrick Couvreur
Jul 27, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Elise LepeltierPatrick Couvreur
Oct 11, 2015·Journal of Drug Targeting·Mehdi Kazemzadeh-NarbatAli Khademhosseini
Jan 16, 2017·Molecular Aspects of Medicine·Maria PeleliMattias Carlström
Oct 2, 2017·Archives of Toxicology·Tzutzuy RamirezBen van Ravenzwaay
Feb 7, 2018·Drug Discovery Today·Vibhuti Agrahari, Vivek Agrahari
Jun 1, 2018·Physiological Reviews·Pier Andrea BoreaKatia Varani

❮ Previous
Next ❯

Citations

Sep 26, 2021·International Journal of Pharmaceutics·Marie CaillaudLiliane Massaad-Massade

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Pharmaceutics
L BildsteinPatrick Couvreur
European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V
Melisande HolzerKlaus Langer
© 2022 Meta ULC. All rights reserved