Towards a complete picture of splice variants of the gene for 25-hydroxyvitamin D31alpha-hydroxylase in brain and skin cancer

The Journal of Steroid Biochemistry and Molecular Biology
Britta DieselEckart Meese

Abstract

Recently, we reported amplification of the gene encoding the P450 Cytochrome 25-hydroxyvitamin D(3)1alpha-hydroxylase (CYP27B1) in 25% of human malignant glioma. Additionally, we reported the first alternative splice variants of CYP27B1. Here, we developed and employed a highly specific approach that combined nested and touchdown PCR to clone full length CYP27B1. In addition, we identified several new splice variants in human melanoma, glioblastoma multiforme (GBM), cervix carcinoma and kidney cell lines. All of the examined cell lines showed a similar expression pattern of the CYP27B1 variants. The new splice variants that were termed Hyd-V5, -V6, -V7, and -V8 were cloned and sequenced. All but one of the new variants showed an insertion of intron 1 leading to a premature termination signal and to truncated proteins without ferredoxin and haem-binding site of the P450 protein. There was no influence of 1alpha,25(OH)(2)D(3) on the expression pattern of the splice variants in melanoma cell line SkMel28.

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Citations

Aug 28, 2007·Nature Reviews. Cancer·Kristin K DeebCandace S Johnson
Jan 24, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Steffi BeckerMichael Friedrich
Feb 9, 2007·The Journal of Steroid Biochemistry and Molecular Biology·T CordesM Friedrich
Sep 10, 2015·International Journal of Alzheimer's Disease·Anindita BanerjeeSasanka Chakrabarti
Nov 26, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Christina KrämerJörg Reichrath
Feb 12, 2017·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Andrew J AnnaloraPatrick L Iversen
Feb 1, 2015·Melanoma Management·Candy WyattRobyn M Lucas
Aug 3, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Britta DieselEckart Meese

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