Towards a molecular identification and classification system of lepidopteran-specific baculoviruses

Virology
Martin LangeJohannes A Jehle

Abstract

Virus genomics provides novel approaches for virus identification and classification. Based on the comparative analyses of sequenced lepidopteran-specific baculovirus genomes, degenerate oligonucleotides were developed that allow the specific amplification of several regions of the genome using polymerase chain reaction (PCR) followed by DNA sequencing. The DNA sequences within the coding regions of three highly conserved genes, namely polyhedrin/granulin (polh/gran), late expression factor 8 (lef-8), and late expression factor 9 (lef-9), were targeted for amplification. The oligonucleotides were tested on viral DNAs isolated from historical field samples, and amplification products were generated from 12 isolated nucleopolyhedrovirus (NPV) and 8 granulovirus (GV) DNAs. The PCR products were cloned or directly sequenced, and phylogenetic trees were inferred from individual and combined data sets of these three genes and compared to a phylogeny, which includes 22 baculoviruses using a combined data set of 30 core genes. This method allows a fast and reliable detection and identification of lepidopteran-specific NPVs and GVs. Furthermore, a strong correlation of the base composition of these three genome areas with that of the co...Continue Reading

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Citations

Jul 10, 2007·International Journal of Environmental Research and Public Health·Mohamed-Bassem AshourBruce D Hammock
Mar 1, 2011·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Shu-Bo ZhangJian-Huang Lai
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Dec 18, 2013·Journal of Virology·Manli WangHualin Wang
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