Towards a more predictable plant breeding pipeline with CRISPR/Cas-induced allelic series to optimize quantitative and qualitative traits

Current Opinion in Plant Biology
Armin Scheben, David Edwards

Abstract

The rate of crop improvement must increase to meet rising global demand for food. Crop breeding pipelines can be hampered by the imprecision and multi-generational timeframe of methods such as intercrossing and stochastic mutagenesis used to generate variation. Genome editing can generate targeted allelic series of trait-related genes and regulatory elements, creating a series of variable phenotypes for breeding within a single generation. Disrupting genic and regulatory regions is particularly effective for engineering quantitative traits. Although qualitative traits can be more difficult to engineer using disruption, precise base editing may allow an efficient path to rationally improve qualitative traits if protein function can be accurately modelled. As functional understanding of genes and regulatory elements increases, genome editing can enhance the predictability of plant breeding outcomes and will ensure food security.

Citations

Dec 1, 2018·The Plant Journal : for Cell and Molecular Biology·Anthony M BolgerBjörn Usadel
Jan 11, 2020·The Plant Journal : for Cell and Molecular Biology·Paul D FraserAlisdair R Fernie
Jul 16, 2020·Genes·Armin Scheben, Diego Hojsgaard
Sep 13, 2018·Plants·Arun S K ShunmugamLinda Y Gorim
May 1, 2019·Annual Review of Plant Biology·Munenori Kitagawa, David Jackson
Oct 28, 2018·Genome Biology·Armin Scheben, David Edwards
Jul 25, 2019·Nature Biotechnology·Benjamin W ThuronyiDavid R Liu
Jun 14, 2019·Frontiers in Bioengineering and Biotechnology·Siguna Mueller
Jun 27, 2019·Planta·Sonali MohapatraHrudayanath Thatoi
Jan 10, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Karen MasselIan D Godwin
Jan 6, 2021·International Journal of Molecular Sciences·Aldrin Y CantilaJacqueline Batley
Jul 19, 2019·Journal of Microbiology & Biology Education·Nicholas J RuppelMichael J Wolyniak

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