Towards a novel haemoglobin-based oxygen carrier: Euro-PEG-Hb, physico-chemical properties, vasoactivity and renal filtration

Biochimica Et Biophysica Acta
I PortöroA Eke

Abstract

Blood transfusion is still a critical therapy in many diseases, traumatic events and war battlefields. However, blood cross-matching and storage may limit its applicability, especially in Third World countries. Moreover, haemoglobin, which in red blood cells is the key player in the oxygen transport from lung to tissues, when free in the plasma causes hypertension and renal failure. This investigation was aimed at the development of a novel haemoglobin-based oxygen carrier with low vasoactivity and renal filtration properties. Human haemoglobin was chemically conjugated with polyethylene glycol (PEG) under either aerobic or anaerobic conditions, following different chemical procedures. The resulting PEGylated haemoglobin products were characterized in terms of oxygen affinity, cooperativity, effects of protons and carbon dioxide concentration, and oxidation stability, and were transfused into rats to evaluate vasoactivity and renal filtration. A deoxyhaemoglobin, conjugated with seven PEG and seven propionyl groups, which we called Euro-PEG-Hb, did not produce profound hypertension, was 99% retained within 6 h, and exhibited oxygen binding properties and allosteric effects more similar to human haemoglobin A than the other test...Continue Reading

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Citations

Mar 15, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jin Hyen BaekPaul W Buehler
Mar 15, 2011·Biochimica Et Biophysica Acta·Martin K SafoTelih Boyiri
Sep 8, 2010·Analytical Biochemistry·Luca RondaAndrea Mozzarelli
Jul 15, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Clémence Le CœurStéphane Longeville
Nov 1, 2016·Biotechnology Progress·Ying WangHong Zhou
Aug 17, 2019·Drug Development and Industrial Pharmacy·Ahmed A H Abdellatif
Feb 20, 2013·Clinical Chemistry and Laboratory Medicine : CCLM·Luca RondaGiuseppe Lippi
May 17, 2017·Pharmaceutical Research·Luca RondaStefano Bettati
Jun 17, 2020·Biomaterials Science·Chris E CooperBrandon J Reeder
Jan 25, 2013·Artificial Cells, Nanomedicine, and Biotechnology·Florina ScurtuRadu Silaghi-Dumitrescu
Sep 22, 2020·Bioinorganic Chemistry and Applications·Anca D FarcasRadu Silaghi-Dumitrescu
Jun 19, 2009·Bioconjugate Chemistry·Dario CacciaAndrea Mozzarelli
Oct 6, 2010·Archives of Biochemistry and Biophysics·Serena FaggianoAndrea Mozzarelli

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