Abstract
A rapid test to identify patients with sickle cell disease could have important benefits in low-resource settings. Sickle cell anemia (SCA) affects about 300,000 newborns each year, the majority of whom are born in sub-Saharan Africa. Low-cost therapies are available to treat SCA, but most countries in sub-Saharan Africa lack robust neonatal screening programs needed to identify patients in need of treatment. To address this need, we developed and evaluated a competitive lateral flow assay that identifies patients with SCA (genotype HbSS) in 15 minutes using undiluted whole blood. A small volume of blood (0.5 μL- 3 μL) is mixed with antibody-coated blue latex beads in a tube and applied to the strip. Strips are then placed in a well of running buffer and allowed to run for 10 minutes. Laboratory evaluation with samples containing different proportions of hemoglobin A (HbA) and hemoglobin S (HbS) indicated that the test should enable identification of SCA patients but not persons with sickle cell trait (SCT). We evaluated the test using 41 samples from individuals with SCA, SCT, and normal blood. With visual inspection or quantitative analysis, we found a 98% accuracy when differentiating SCA from normal and SCT samples as a gro...Continue Reading
References
Jan 1, 1975·Clinical Pharmacology and Therapeutics·A R Feinstein
Jul 6, 1992·Journal of Immunological Methods·S A Fernando, G S Wilson
Dec 1, 1968·Clinica Chimica Acta; International Journal of Clinical Chemistry·R N WrightstoneA van der Sar
Jan 1, 1959·British Medical Bulletin·J C WHITE, G H BEAVEN
Apr 15, 2008·Analytical Chemistry·Andres W MartinezGeorge M Whitesides
Mar 25, 2011·Trends in Parasitology·Thomas N Williams, Stephen K Obaro
Oct 1, 2011·Malaria Journal·Jennifer LuchavezDavid Bell
Apr 27, 2012·Pediatric Blood & Cancer·Banu Aygun, Isaac Odame
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Oct 30, 2012·Lancet·Frédéric B PielSimon I Hay
Feb 23, 2013·Lab on a Chip·Xiaoxi YangSergey S Shevkoplyas
Jul 15, 2015·Molecular Reproduction and Development·Johannes SchindelinKevin W Eliceiri
Sep 18, 2015·BMC Medicine·Julie KanterXiaoxi Yang
Nov 6, 2015·American Journal of Hematology·Patrick T McGannRussell E Ware
Jan 7, 2016·PloS One·Nathaniel Z PietySergey S Shevkoplyas
Sep 9, 2016·British Journal of Haematology·Charles T QuinnMark Geisberg
Citations
Aug 21, 2018·Current Opinion in Pediatrics·Bradford L Therrell, Carmencita D Padilla
Jun 9, 2020·NPJ Digital Medicine·Kevin de HaanAydogan Ozcan
Feb 19, 2021·NPJ Digital Medicine·Kevin de HaanAydogan Ozcan
Jul 31, 2020·Biosensors & Bioelectronics·Shazia IlyasWaseem Asghar
Jun 3, 2021·Micromachines·Wjdan A ArishiMohammed Zourob
Sep 28, 2018·Analytical Chemistry·Hua Li, Andrew J Steckl