PMID: 9178493May 1, 1997Paper

Towards defining a minimal functional domain for NTPase and RNA helicase activities of the hepatitis C virus NS3 protein

Virus Research
D W KimJ Choe

Abstract

Hepatitis C virus (HCV) possesses two separate enzymatic functions in the NS3 protein: a protease and an NTPase/RNA helicase. In order to determine the minimal domain for NTPase and RNA helicase activities of the HCV NS3 protein, serial deletion mutants were constructed. The NS3H protein a fusion protein of 25 amino acids (aa) from an expression vector and the C-terminal 466 aa of the HCV NS3 protein, contains an NTPase/RNA helicase activity. We made deletion mutants of 10, 30, 50, 97, and 135 aa from the C-terminus and 16 and 32 aa from the N-terminus of the NS3H protein. The deleted protein lacking 50 aa from the C-terminus still possessed both activities, while the protein lacking 97 aa from the C-terminus lost an RNA helicase activity. The mutant lacking 16 amino acids from the N-terminus retained the enzymatic activities and the N-terminal 32 aa deleted mutant lost an NTPase/RNA helicase activity. A combinational deletion mutant lacking 16 aa the N-terminus and 50 aa from the C-terminus retained the enzymatic activities. These results show that the functional domain of the HCV NTPase/ RNA helicase is about 400 aa in length and maps between NS3 residues 1209 and 1608.

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·Q L ChooP J Barr
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M HijikataK Shimotohno
Jan 11, 2005·Archives of Biochemistry and Biophysics·Khondaker M NasirudinNarendra Tuteja

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Citations

Jun 27, 2000·Antiviral Research·M Hicham Alaoui-IsmailiJ Bedard
Sep 11, 2002·Antiviral Research·Peter BorowskiHerbert Schmitz
Apr 5, 2005·Nature Structural & Molecular Biology·Mikhail K LevinSmita S Patel
Jul 1, 1999·Protein Science : a Publication of the Protein Society·A Y HoweJ Y Lau
Jan 11, 2000·Clinical Microbiology Reviews·P LeyssenJ Neyts
May 30, 2006·Protein Science : a Publication of the Protein Society·Vikas JainDipankar Chatterji
Dec 14, 2011·Journal of Molecular Biology·Margaret E Fairman-Williams, Eckhard Jankowsky
Jun 7, 2003·Antiviral Chemistry & Chemotherapy·Michelle P WalkerZhi Hong
Nov 26, 1997·Journal of Virology·D W KimJ Choe

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