Towards designer cellulosomes in Clostridia: mannanase enrichment of the cellulosomes produced by Clostridium cellulolyticum

Journal of Bacteriology
Stéphanie PerretChantal Tardif

Abstract

The man5K gene of Clostridium cellulolyticum was cloned and overexpressed in Escherichia coli. This gene encodes a 424-amino-acid preprotein composed of an N-terminal leader peptide, followed by a dockerin module and a C-terminal catalytic module belonging to family 5 of the glycosyl hydrolases. Mature Man5K displays 62% identity with ManA from Clostridium cellulovorans. Two forms of the protein were purified from E. coli; one form corresponds to the full-length enzyme (45 kDa), and a truncated form (39 kDa) lacks the N-terminal dockerin module. Both forms exhibit the same typical family 5 mannanase substrate preference; they are very active with the galactomannan locust bean gum, and the more galacto-substituted guar gum molecules are degraded less. The truncated form, however, displays fourfold-higher activity with galactomannans than the full-length enzyme. Man5K was successfully overproduced in C. cellulolyticum by using expression vectors. The trans-produced protein was found to be incorporated into the cellulosomes and became one of the major enzymatic components. Modified cellulosomes displayed 20-fold-higher specific activities than control fractions on galactomannan substrates, whereas the specific activity on crystall...Continue Reading

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Feb 6, 2004·Molecular Microbiology·Stéphanie PerretChantal Tardif

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Citations

Jan 1, 2009·Biotechnology Letters·Robert E NordonFrances C Foong
May 4, 2010·Applied and Environmental Microbiology·Jean-Charles BlouzardPascale de Philip
Mar 5, 2005·Applied and Environmental Microbiology·Florence MingardonHenri-Pierre Fierobe
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Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Makiko SakkaKazuo Sakka
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