Towards functional 3D-stacked electrospun composite scaffolds of PHBV, silk fibroin and nanohydroxyapatite: Mechanical properties and surface osteogenic differentiation

Journal of Biomaterials Applications
Elena I PaşcuGarrett B McGuinness

Abstract

Bone tissue engineering scaffolds have two challenging functional tasks to fulfil: to encourage cell proliferation, differentiation and matrix synthesis and to provide suitable mechanical stability upon implantation. Composites of biopolymers and bioceramics combine the advantages of both types of materials, resulting in better processability and enhanced mechanical and biological properties through matrix reinforcement. In the present study, novel thick bone composite scaffolds were successfully fabricated using electrospun flat sheets of polyhydroxybutyrate-polyhydroxyvalerate/nanohydroxyapatite/silk fibroin essence (2% nanohydroxyapatite - 2% silk fibroin essence and 5% nanohydroxyapatite - 5% silk fibroin essence, respectively). Their potential asin vitrobone regeneration scaffolds was evaluated using mouse calvarian osteoblast cells (MC3T3-E1), in terms of morphology (scanning electron microscope), cell attachment, cell proliferation, Col type I, osteopontin and bone alkaline phosphatase activity (Quantitative Real Time Polymerase Chain Reaction [qRT-PCR], enzyme-linked immunosorbent assay, immunocytochemistry). Electrospun polyhydroxybutyrate-polyhydroxyvalerate scaffolds were used as reference constructs. The results sho...Continue Reading

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Oct 8, 2013·Materials Science & Engineering. C, Materials for Biological Applications·Elena I PaşcuGarrett B McGuinness

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Citations

Dec 10, 2016·Journal of Biomaterials Science. Polymer Edition·Bingjie NiuLiang Chen
Sep 28, 2018·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Tony YuMichele Marcolongo
May 13, 2019·ACS Biomaterials Science & Engineering·Shreya MehrotraBiman B Mandal

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enzyme-linked immunosorbent assay
confocal microscopy

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