Towards functional glycomics by lectin histochemistry: strategic probe selection to monitor core and branch-end substitutions and detection of cell-type and regional selectivity in adult mouse testis and epididymis

Anatomia, histologia, embryologia
Michaela LohrH J Gabius

Abstract

The emerging insights into glycan functionality direct increasing attention to monitor core modifications of N-glycans and branch-end structures. To address this issue in histochemistry, a panel of lectins with respective specificities was devised. The selection of probes with overlapping specificities facilitated to relate staining profiles to likely target structures. The experiments on fixed sections of adult murine testis and epididymis were carried out at non-saturating lectin concentrations to visualize high-affinity sites with optimal signal-to-background ratio. They revealed selectivity in lectin reactivity for distinct cell types and segment-dependent staining in the epididymis. Leydig cells, for instance, were reactive with the Sambucus nigra agglutinin and human siglec-2 (CD22), two lectins also separating principal from basal and apical cells in the caput segments I-III of the epididymis. Apical cells were reactive with the Maackia amurensis agglutinin-I, and basal cells with the erythroagglutinin of Phaseolus vulgaris. The reported differences support the concept of lectin staining as cell marker. They thus intimate to study glycogene (genes for glycosyltransferases and lectins) expression and cellular reactivity w...Continue Reading

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Citations

Apr 5, 2011·Trends in Biochemical Sciences·Hans-Joachim GabiusDolores Solís
Mar 10, 2016·Cellular and Molecular Life Sciences : CMLS·H-J GabiusH Kaltner
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Mar 7, 2017·Scientific Reports·Xia ZouAtsushi Kuno
Jan 27, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sabine AndréHans-Joachim Gabius
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Jun 25, 2019·Frontiers in Cell and Developmental Biology·Ayodele Akintayo, Pamela Stanley
Dec 22, 2016·Histochemistry and Cell Biology·Jürgen Kopitz
Dec 26, 2016·Histochemistry and Cell Biology·Joachim C ManningHans-Joachim Gabius
Feb 3, 2021·Biomolecules·Zorana LopandićMarija Gavrović-Jankulović
Dec 25, 2010·Nitric Oxide : Biology and Chemistry·Marlies Van de WouwerAntonio Villalobo

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