Towards genome-wide experimental genetics in the in vivo malaria model parasite Plasmodium berghei

Pathogens and Global Health
Joachim M Matz, Taco W A Kooij

Abstract

Plasmodium berghei was identified as a parasite of thicket rats (Grammomys dolichurus) and Anopheles dureni mosquitoes in African highland forests. Successful adaptation to a range of rodent and mosquito species established P. berghei as a malaria model parasite. The introduction of stable transfection technology, permitted classical reverse genetics strategies and thus systematic functional profiling of the gene repertoire. In the past 10 years following the publication of the P. berghei genome sequence, many new tools for experimental genetics approaches have been developed and existing ones have been improved. The infection of mice is the principal limitation towards a genome-wide repository of mutant parasite lines. In the past few years, there have been some promising and most welcome developments that allow rapid selection and isolation of recombinant parasites while simultaneously minimising animal usage. Here, we provide an overview of all the currently available tools and methods.

References

Apr 1, 1986·Annals of Tropical Medicine and Parasitology·G E Childs, C Lambros
Jun 2, 1995·Science·M R van DijkC J Janse
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y WuT E Wellems
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R G Donald, D S Roos
Jul 1, 1993·The American Journal of Tropical Medicine and Hygiene·C J CanfieldD P Jacobus
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R GoonewardeneD Wirth
Apr 21, 1997·The Journal of Experimental Medicine·A M van der WelA W Thomas
Jun 1, 1997·Molecular and Biochemical Parasitology·M R van DijkC J Janse
Feb 12, 1998·Methods : a Companion to Methods in Enzymology·A P WatersC J Janse
Mar 19, 1999·Molecular and Cellular Biology·A NunesR Ménard
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·C B MamounD E Goldberg
Apr 9, 2001·The Journal of Biological Chemistry·R M van SpaendonkA P Waters
Apr 11, 2001·Molecular and Biochemical Parasitology·M M MotaV Nussenzweig
Oct 9, 2002·Nature·Malcolm J GardnerBart Barrell
Apr 22, 2003·Nature Structural Biology·Jirundon YuvaniyamaYongyuth Yuthavong
Jun 10, 2004·Cellular Microbiology·Friedrich FrischknechtRobert Ménard
Jul 29, 2004·Molecular and Biochemical Parasitology·Blandine Franke-FayardChris J Janse
Oct 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Teresa Gil CarvalhoRobert Ménard
Jun 10, 2005·The EMBO Journal·Viswanathan LakshmananDavid A Fidock
Aug 24, 2005·Molecular and Biochemical Parasitology·Taco W A KooijAndrew P Waters
Oct 26, 2005·Molecular and Biochemical Parasitology·Chris J JanseAndrew P Waters
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Bharath BaluJohn H Adams
Nov 15, 2005·Nucleic Acids Research·Hiroshi SakamotoRobert Ménard
Jan 24, 2006·Nature Medicine·Rogerio AminoRobert Ménard
May 24, 2006·Molecular and Biochemical Parasitology·Judith M PfahlerMichael Lanzer
Aug 8, 2006·International Journal for Parasitology·Alice S TarunStefan H I Kappe
Jan 9, 2008·Trends in Parasitology·Rachanee UdomsangpetchJetsumon Sattabongkot
Feb 5, 2008·Molecular and Biochemical Parasitology·Sebastian A MikolajczakStefan H I Kappe
May 3, 2008·Molecular Microbiology·Victoria CarterJohannes T Dessens
Jun 14, 2008·PLoS Pathogens·Christiaan van OoijKasturi Haldar
Oct 10, 2008·Nature·Jane M CarltonClaire M Fraser-Liggett

❮ Previous
Next ❯

Citations

Jan 23, 2016·Nature Communications·Sanketha KenthirapalanTaco W A Kooij
May 19, 2018·FEBS Letters·Akira SogaShinya Fukumoto
Mar 31, 2015·Eukaryotic Cell·Joachim M MatzTaco W A Kooij
Dec 5, 2018·NPJ Vaccines·António M MendesMiguel Prudêncio
Jun 5, 2020·Journal of Prosthodontics : Official Journal of the American College of Prosthodontists·Georgi TalmazovRami Ammoun
Jul 1, 2020·Proceedings of the National Academy of Sciences of the United States of America·Joachim M MatzMichael J Blackman
May 16, 2018·Antimicrobial Agents and Chemotherapy·Hong-Bin YanGabriel Rinaldi
Sep 26, 2020·Cellular Microbiology·Francois KorbmacherJoachim M Matz
Feb 16, 2021·Frontiers in Cellular and Infection Microbiology·Annemarie Voorberg-van der WelAnne-Marie Zeeman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.