Towards industrial usefulness - cryo-cell-banking of transgenic BY-2 cell cultures

Journal of Biotechnology
Kathrin SchmaleStephan Hellwig

Abstract

Plants have been established as an useful production system for commercially relevant proteins. Plant cell cultures show certain advantages compared to field-grown plants. However, one critical drawback for the long-term use of recombinant plant cell cultures is the instability of cell cultures concerning genetic background and productivity when maintained by subculturing. The ability to store recombinant cell lines stably by cryopreservation allows to maintain an efficient and stable production system. In this work, we describe the development of a cryopreservation protocol for a transgenic BY-2 cell culture expressing human serum albumin. In 1.75-L stirred-tank bioreactors growth and production kinetics of the transgenic cell line were compared after cryopreservation to the hitherto performed maintenance by subculturing. Growth and productivity of the cryopreserved cell culture remained stable after freezing for one week. Here, we show that we developed an efficient method which allows the storage of transgenic plant cell cultures, an important requirement for industrial processes.

References

Oct 1, 1974·Cryobiology·D K Dougall, D F Wetherell
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Nov 20, 2003·Trends in Biotechnology·Richard M TwymanRainer Fischer
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Mar 9, 2004·Current Opinion in Plant Biology·Rainer FischerRichard M Twyman
Nov 6, 2004·Nature Biotechnology·Stephan HellwigRainer Fischer

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Citations

Nov 15, 2011·Biotechnology Advances·Biao WangQiaochun Wang
Aug 19, 2011·Biotechnology Advances·Ting-Kuo Huang, Karen A McDonald
Jan 18, 2011·Biotechnology Advances·Jianfeng XuMaureen C Dolan
Mar 26, 2016·Frontiers in Plant Science·Rita B SantosTanja Holland
Dec 18, 2013·Plant Biotechnology Journal·Lauri J ReuterAnneli Ritala
Oct 18, 2016·Frontiers in Plant Science·Suvi T HäkkinenKirsi-Marja Oksman-Caldentey
Apr 9, 2008·Advances in Applied Microbiology·Sarad ParekhMichael Horn
Jul 3, 2015·PloS One·Vivek Phani VarmaSandeep Goel
Sep 29, 2006·Biotechnology and Bioengineering·Bénédicte TerrierVincent Pétiard

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